Literature DB >> 26855363

epiGBS: reference-free reduced representation bisulfite sequencing.

Thomas P van Gurp1, Niels C A M Wagemaker2, Björn Wouters1, Philippine Vergeer3, Joop N J Ouborg2, Koen J F Verhoeven1.   

Abstract

We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.

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Year:  2016        PMID: 26855363     DOI: 10.1038/nmeth.3763

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


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