Rosaria M Ruggeri1, Teresa M Vicchio1, Mariateresa Cristani2, Rosaria Certo1, Daniela Caccamo3, Angela Alibrandi4, Salvatore Giovinazzo1, Antonina Saija2, Alfredo Campennì3, Francesco Trimarchi1,5, Sebastiano Gangemi6. 1. 1 Department of Clinical and Experimental Medicine, Division of Endocrinology, University of Messina , Messina, Italy . 2. 2 Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina , Messina, Italy . 3. 3 Department of Biomedical Sciences and Morpho-Functional Imaging, University of Messina , Messina, Italy . 4. 4 Department of Economics, University of Messina , Messina, Italy . 5. 5 Accademia Peloritana dei Pericolanti, University of Messina , Messina, Italy . 6. 6 School and Division of Allergy and Clinical Immunology, Department of Clinical and Experimental Medicine, University of Messina , Messina, Italy .
Abstract
BACKGROUND: Oxidative stress, which occurs as a result of an imbalance between free-radical production and antioxidant defense mechanisms, has been implicated in the pathogenesis of several autoimmune disorders, including thyroid diseases. Importantly, it has been correlated to thyroid dysfunction. This study investigated the changes in oxidative balance in euthyroid Hashimoto's thyroiditis (HT) by means of specific serum tests, such as derived reactive oxygen metabolites (d-ROMs) and the biological antioxidant potential (BAP) test. In addition, advanced glycation end products (AGEs) and advanced oxidation protein products (AOPPs)--compounds formed by the transformation of proteins--were evaluated as potential new markers of oxidative stress in this disease. METHODS: This study included 134 euthyroid subject: 71 newly diagnosed HT patients (63 females; M age = 38 ± 13 years) and 63 age and sex-matched healthy controls. None of them were on thyroxine therapy. RESULTS: Serum d-ROMs were elevated, and BAP decreased in HT patients compared with controls (p < 0.001), and the two parameters were inversely correlated (r = -0.211; p = 0.027), clearly indicating an enhanced oxidative stress. Furthermore, AGE levels were higher in HT patients (M = 223.18 AU/g prot) than in controls (M = 189.636 AU/g prot; p = 0.020) and inversely correlated with BAP levels (r = -0.196; p = 0.037). In uni- and multivariate analysis, serum antithyroperoxidase antibodies were the main predictors for d-ROMs (p = 0.006), BAP (p < 0.001), and AGEs (p = 0.014), irrespective of thyrotropin and/or free thyroxine values. No differences in AOPPs levels were found between patients and controls (p = 0.923). CONCLUSIONS: Oxidants are increased and antioxidants decreased in euthyroid HT patients. As a result, the oxidative/antioxidative balance is shifted toward the oxidative side. Moreover, this study reports on a possible significant involvement of AGEs in HT, thus contributing to a better definition of the redox homoeostasis dysregulation in HT.
BACKGROUND:Oxidative stress, which occurs as a result of an imbalance between free-radical production and antioxidant defense mechanisms, has been implicated in the pathogenesis of several autoimmune disorders, including thyroid diseases. Importantly, it has been correlated to thyroid dysfunction. This study investigated the changes in oxidative balance in euthyroid Hashimoto's thyroiditis (HT) by means of specific serum tests, such as derived reactive oxygen metabolites (d-ROMs) and the biological antioxidant potential (BAP) test. In addition, advanced glycation end products (AGEs) and advanced oxidation protein products (AOPPs)--compounds formed by the transformation of proteins--were evaluated as potential new markers of oxidative stress in this disease. METHODS: This study included 134 euthyroid subject: 71 newly diagnosed HTpatients (63 females; M age = 38 ± 13 years) and 63 age and sex-matched healthy controls. None of them were on thyroxine therapy. RESULTS: Serum d-ROMs were elevated, and BAP decreased in HTpatients compared with controls (p < 0.001), and the two parameters were inversely correlated (r = -0.211; p = 0.027), clearly indicating an enhanced oxidative stress. Furthermore, AGE levels were higher in HTpatients (M = 223.18 AU/g prot) than in controls (M = 189.636 AU/g prot; p = 0.020) and inversely correlated with BAP levels (r = -0.196; p = 0.037). In uni- and multivariate analysis, serum antithyroperoxidase antibodies were the main predictors for d-ROMs (p = 0.006), BAP (p < 0.001), and AGEs (p = 0.014), irrespective of thyrotropin and/or free thyroxine values. No differences in AOPPs levels were found between patients and controls (p = 0.923). CONCLUSIONS: Oxidants are increased and antioxidants decreased in euthyroid HTpatients. As a result, the oxidative/antioxidative balance is shifted toward the oxidative side. Moreover, this study reports on a possible significant involvement of AGEs in HT, thus contributing to a better definition of the redox homoeostasis dysregulation in HT.
Authors: R M Ruggeri; M Cristani; T M Vicchio; A Alibrandi; S Giovinazzo; A Saija; A Campennì; F Trimarchi; S Gangemi Journal: J Endocrinol Invest Date: 2018-05-23 Impact factor: 4.256
Authors: G Rotondo Dottore; I Ionni; F Menconi; G Casini; S Sellari-Franceschini; M Nardi; P Vitti; C Marcocci; M Marinò Journal: J Endocrinol Invest Date: 2017-12-18 Impact factor: 4.256
Authors: Aniceta A Mikulska; Marta Karaźniewicz-Łada; Dorota Filipowicz; Marek Ruchała; Franciszek K Główka Journal: Int J Mol Sci Date: 2022-06-13 Impact factor: 6.208