Rodrigo Neves-Silva1, Felipe Paiva Fonseca1, Adriana Souza de Jesus2, Hélder Antônio Rebelo Pontes2, André Caroli Rocha3, Thais Bianca Brandão1, Pablo Agustin Vargas1,4, Márcio Ajudarte Lopes1, Oslei Paes de Almeida1, Alan Roger Santos-Silva5. 1. Oral Diagnosis Department, Semiology and Pathology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil. 2. Service of Oral Pathology, João de Barros Barreto University Hospital, Federal University of Pará, Belém, Pará, Brazil. 3. Medical School, Clinics Hospital, University of São Paulo, São Paulo, São Paulo, Brazil. 4. Department of Oral Pathology and Oral Biology, School of Dentistry, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. 5. Oral Diagnosis Department, Semiology and Pathology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil. alanroger@fop.unicamp.br.
Abstract
BACKGROUND: Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. METHODS: Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). RESULTS: Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). CONCLUSION: Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
BACKGROUND:Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different humanneoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. METHODS: Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). RESULTS: Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). CONCLUSION: Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
Authors: Nicole C M Visser; Anneke A M van der Wurff; Johanna M A Pijnenborg; Leon F A G Massuger; Johan Bulten; Iris D Nagtegaal Journal: Virchows Arch Date: 2018-02-09 Impact factor: 4.064
Authors: Katherine Julissa Palma Valladares; Karolyny Martins Balbinot; Antonia Taiane Lopes de Moraes; Maria Sueli da Silva Kataoka; Aline Maria Pereira Cruz Ramos; Rommel Thiago Jucá Ramos; Artur Luiz da Costa da Silva; Ricardo Alves Mesquita; David Normando; Sérgio de Melo Alves Júnior; João de Jesus Viana Pinheiro Journal: Int J Dent Date: 2021-12-27