| Literature DB >> 26836019 |
M J Vilar1, B Peralta2, I García-Bocanegra3, M Simon-Grifé2, A Bensaid2, J Casal4, J Segalés4, S Pina-Pedrero5.
Abstract
The prevalence of Enterovirus G (EV-G) and Sapelovirus A (PSV-1) was investigated in Spanish swine herds by means of cross-sectional studies. Faecal samples from clinically healthy pigs were collected from six farms, and analysed by RT-PCR. The results indicated a high prevalence of EV-G detected in nearly all the animals older than 3 weeks of age. Otherwise, PSV-1 was only detected in 3-week-old piglets from one of the farms. Genetic analyses performed in the VP1 region of the EV-G indicated circulation of diverse strains in the same farm, related to genotypes G1, G2, G3, G4, G6, G9, G12, G13 and G14. Moreover, co-infection of several PSV-1 variants in the same animal was evident, typical of viral quasispecies. Evolutionary pressure analysis indicated that microevolution of PSV-1 seems to be driven by negative selection. This study gives further insights in the epidemiology of EV-G and PSV-1.Entities:
Keywords: PCR; Phylogenetic analysis; Quasispecies
Mesh：
Year: 2016 PMID： 26836019 DOI： 10.1016/j.virusres.2016.01.019
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303