Mohammad Hossein Geranmayeh1, Ali Baghbanzadeh1, Abbas Barin2, Jamileh Salar-Amoli3, Mohammad Mehdi Dehghan4, Reza Rahbarghazi5, Hassan Azari6. 1. Section of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 2. Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 3. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 4. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 5. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 6. Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences, Shiraz School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Neural Stem Cell and Regenerative Neuroscience Laboratory, Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
PURPOSE: Glutamate is a major excitatory neurotransmitter in mammalian central nervous system. Excessive glutamate releasing overactivates its receptors and changes calcium homeostasis that in turn leads to a cascade of intracellular events causing neuronal degeneration. In current study, we used neural stem cells conditioned medium (NSCs-CM) to investigate its neuroprotective effects on glutamate-treated primary cortical neurons. METHODS: Embryonic rat primary cortical cultures were exposed to different concentrations of glutamate for 1 hour and then they incubated with NSCs-CM. Subsequently, the amount of cell survival in different glutamate excitotoxic groups were measured after 24 h of incubation by trypan blue exclusion assay and MTT assay. Hoechst and propidium iodide were used for determining apoptotic and necrotic cell death pathways proportion and then the effect of NSCs-CM was investigated on this proportion. RESULTS: NSCs conditioned medium increased viability rate of the primary cortical neurons after glutamate-induced excitotoxicity. Also we found that NSCs-CM provides its neuroprotective effects mainly by decreasing apoptotic cell death rate rather than necrotic cell death rate. CONCLUSION: The current study shows that adult neural stem cells could exert paracrine neuroprotective effects on cortical neurons following a glutamate neurotoxic insult.
PURPOSE:Glutamate is a major excitatory neurotransmitter in mammalian central nervous system. Excessive glutamate releasing overactivates its receptors and changes calcium homeostasis that in turn leads to a cascade of intracellular events causing neuronal degeneration. In current study, we used neural stem cells conditioned medium (NSCs-CM) to investigate its neuroprotective effects on glutamate-treated primary cortical neurons. METHODS: Embryonic rat primary cortical cultures were exposed to different concentrations of glutamate for 1 hour and then they incubated with NSCs-CM. Subsequently, the amount of cell survival in different glutamate excitotoxic groups were measured after 24 h of incubation by trypan blue exclusion assay and MTT assay. Hoechst and propidium iodide were used for determining apoptotic and necrotic cell death pathways proportion and then the effect of NSCs-CM was investigated on this proportion. RESULTS: NSCs conditioned medium increased viability rate of the primary cortical neurons after glutamate-induced excitotoxicity. Also we found that NSCs-CM provides its neuroprotective effects mainly by decreasing apoptotic cell death rate rather than necrotic cell death rate. CONCLUSION: The current study shows that adult neural stem cells could exert paracrine neuroprotective effects on cortical neurons following a glutamateneurotoxic insult.
Authors: Beatriz Martínez-Rojas; Esther Giraldo; Rubén Grillo-Risco; Marta R Hidalgo; Eric López-Mocholi; Ana Alastrue-Agudo; Francisco García-García; Victoria Moreno-Manzano Journal: Cell Mol Life Sci Date: 2022-07-29 Impact factor: 9.207
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