| Literature DB >> 26807702 |
Marta P B Mourão1, Sjoukje Kuijper2, Ngoc A Dang2, Elisabetta Walters3, Hans-Gerd Janssen4, Arend H J Kolk2.
Abstract
Tuberculosis (TB) remains a worldwide health problem, especially in developing countries. Correct identification of Mycobacterium tuberculosis (MTB) infection is extremely important for providing appropriate treatment and care to patients. Here we describe a solid phase extraction-gas chromatography-mass spectrometry method (SPE-THM-GC-MS) for the detection of five biomarkers for M. tuberculosis. The method for classification is developed and validated through the analysis of 112 sputum samples from patients suspected of having TB. Twenty of twenty-five MTB culture-positive sputum samples were correctly classified as positive by our improved SPE-THM-GC-MS method. Eighty-five of eighty-seven MTB culture-negative samples were also negative by SPE-THM-GC-MS. The overall sensitivity of the new SPE-THM-GC-MS method is 80% (20/25) and the specificity is 98% (85/87) compared with culture. The method proved to be reliable and, although complex in principle, easy to operate due to the high degree of automation.Entities:
Keywords: Direct detection; Gas chromatography–mass spectrometry; Mycobacterium tuberculosis; Solid Phase Extraction; Validation
Mesh：
Year: 2015 PMID： 26807702 DOI： 10.1016/j.jchromb.2015.12.023
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205