| Literature DB >> 26803635 |
Leonard R Pelgrom1, Alwin J van der Ham1, Bart Everts2.
Abstract
Engagement of Toll-like receptors (TLRs) on dendritic cells (DCs) triggers the expression of a large set of genes involved in DC activation and maturation, which allow them to act efficiently as antigen-presenting cells. Recently, it has become clear that TLR signalling in DCs also results in dramatic metabolic changes that are integral to their changed biology. Here, we describe a detailed protocol on how DC metabolism can be studied after TLR stimulation using the 96-well format Extracellular Flux (XF(e)96) Analyzer from Seahorse Bioscience, a machine that allows one to simultaneously assess rates of oxidative phosphorylation and glycolysis in real-time, in live cells and in a high-throughput manner.Keywords: Cellular bioenergetics; Dendritic cells; Extracellular Flux Analyzer; Extracellular acidification; Glycolysis; Metabolism; Oxidative phosphorylation; Oxygen consumption; Respiration; Seahorse Bioscience; Toll-like receptors
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Year: 2016 PMID： 26803635 DOI： 10.1007/978-1-4939-3335-8_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745