M-C Deng1,2, J Li2,3, Y-H Hong1, X-M Xu1, W-X Chen2, J-P Yuan4, J Peng1, M Yi1, J-H Wang1,4. 1. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, China. 2. Department of Food and Bioengineering, Guangdong Industry Technical College, Guangzhou, China. 3. College of Environment and Energy, South China University of Technology, Guangzhou, China. 4. South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou, China.
Abstract
AIMS: To purify and characterize the biosurfactants produced by Achromobacter sp. HZ01. METHODS AND RESULTS: After fermentation, one biosurfactant was successfully purified from the fermentation broth of strain HZ01 by centrifugation, extraction using ethyl acetate, silica gel chromatography and reversed phase-high performance liquid chromatography. The critical micelle concentration (CMC) of the biosurfactant and the effects of temperatures, pH and salinities on its stability were determined. Fourier transform infrared spectroscopy, analysis of fatty acids and amino acids and mass spectrometry were used to characterize the biosurfactant. The maximum production yield of the crude biosurfactant reached to 6·84 g l(-1) after incubation for 96 h. Except the favourable adaptability to a wide range of temperatures, pH and salinities, the biosurfactant with a CMC value of 48 mg l(-1) could efficiently emulsify diverse hydrophobic compounds. The chemical formula of this biosurfactant was confirmed to be CH3 -(CH2 )17 -CHO-CH2 -CO-Gly-Gly-Leu-Met-Leu-Leu, in which the oxygen atom of group CHO linked to the last amino acid (Leu), a structure had never been reported before. CONCLUSIONS: The purified biosurfactant is a novel cyclic lipopeptide. SIGNIFICANCE AND IMPACT OF THE STUDY: One novel lipopeptide was purified and characterized. The novel biosurfactant exhibited good potential applications, such as bioremediation.
AIMS: To purify and characterize the biosurfactants produced by Achromobacter sp. HZ01. METHODS AND RESULTS: After fermentation, one biosurfactant was successfully purified from the fermentation broth of strain HZ01 by centrifugation, extraction using ethyl acetate, silica gel chromatography and reversed phase-high performance liquid chromatography. The critical micelle concentration (CMC) of the biosurfactant and the effects of temperatures, pH and salinities on its stability were determined. Fourier transform infrared spectroscopy, analysis of fatty acids and amino acids and mass spectrometry were used to characterize the biosurfactant. The maximum production yield of the crude biosurfactant reached to 6·84 g l(-1) after incubation for 96 h. Except the favourable adaptability to a wide range of temperatures, pH and salinities, the biosurfactant with a CMC value of 48 mg l(-1) could efficiently emulsify diverse hydrophobic compounds. The chemical formula of this biosurfactant was confirmed to be CH3 -(CH2 )17 -CHO-CH2 -CO-Gly-Gly-Leu-Met-Leu-Leu, in which the oxygen atom of group CHO linked to the last amino acid (Leu), a structure had never been reported before. CONCLUSIONS: The purified biosurfactant is a novel cyclic lipopeptide. SIGNIFICANCE AND IMPACT OF THE STUDY: One novel lipopeptide was purified and characterized. The novel biosurfactant exhibited good potential applications, such as bioremediation.
Authors: Yoojae Maeng; Kyoung Tae Kim; Xuan Zhou; Litai Jin; Ki Soo Kim; Young Heui Kim; Suyeon Lee; Ji Ho Park; Xiuyu Chen; Mingxia Kong; Lu Cai; Xiaokun Li Journal: Microb Biotechnol Date: 2018-07-18 Impact factor: 5.813
Authors: Naif Abdullah Al-Dhabi; Galal Ali Esmail; Mariadhas Valan Arasu Journal: Int J Environ Res Public Health Date: 2020-11-15 Impact factor: 3.390