Valentina Carito1, Stefania Ciafrè2, Luigi Tarani3, Mauro Ceccanti4, Fausta Natella5, Angela Iannitelli6, Paola Tirassa1, George N Chaldakov7, Marco Ceccanti4, Chiara Boccardo8, Marco Fiore9. 1. Istituto di Biologia Cellulare e Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy. 2. Istituto di Farmacologia Traslazionale, Consiglio Nazionale delle Ricerche, Rome Italy. 3. Dipartimento di Pediatria e Neuropsichiatria Infantile, Sapienza Università di Roma, Rome, Italy. 4. Centro Riferimento Alcologico Regione Lazio, Sapienza Università di Roma, Rome, Italy. 5. Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Rome, Italy. 6. Dipartimento di Scienze e Biotecnologie Medico-Chirurgiche, Facoltà di Farmacia e Medicina, Sapienza Università di Roma, Rome, Italy. 7. Laboratory of Cell Biology, Department of Anatomy and Histology, Medical University, Varna, Bulgaria. 8. Scuola di Specializzazione Medicina di Emergenza-Urgenza, Sapienza Università di Roma, Rome, Italy. 9. Istituto di Biologia Cellulare e Neurobiologia, Consiglio Nazionale delle Ricerche, Rome, Italy - Istituto di Ricovero e Cura a Carattere Scientifico, Fondazione Santa Lucia, Rome, Italy.
Abstract
BACKGROUND: Polyphenols from olive are known to possess antioxidant and anti-inflammatory properties. AIM: The aim of this study was to study whether or not 10 consecutive days i.p. administration of a blend of olive (Olea europaea L.) polyphenols (10 mg/kg) containing mostly hydroxytyrosol could have an effect on cytokines playing important roles in inflammatory processes as TNF-α and IL-10. MATERIALS AND METHODS: Inflammation was induced in the mouse paw by 2 carrageenan injections (50 µl vol, 5 mg/kg each). TNF-α and IL-10 were measured by enzyme-linked immunosorbent assay. RESULTS: Carrageenan decreased IL-10 in the paws, however, this reduction appeared to be less evident in mice treated with carrageenan but administered with polyphenols. As for TNF-α, our findings did not reveal differences between groups but an increase in the polyphenol and carrageenan group if compared to the carrageenan only group. As for antioxidant polyphenols' properties, no differences between groups in the serum glutathione were found. CONCLUSIONS: Altogether, this investigation shows that olive polyphenols in the mouse may modulate the levels of cytokines having a role in the process of inflammation as TNF-α and IL-10.
BACKGROUND:Polyphenols from olive are known to possess antioxidant and anti-inflammatory properties. AIM: The aim of this study was to study whether or not 10 consecutive days i.p. administration of a blend of olive (Olea europaea L.) polyphenols (10 mg/kg) containing mostly hydroxytyrosol could have an effect on cytokines playing important roles in inflammatory processes as TNF-α and IL-10. MATERIALS AND METHODS:Inflammation was induced in the mouse paw by 2 carrageenan injections (50 µl vol, 5 mg/kg each). TNF-α and IL-10 were measured by enzyme-linked immunosorbent assay. RESULTS:Carrageenan decreased IL-10 in the paws, however, this reduction appeared to be less evident in mice treated with carrageenan but administered with polyphenols. As for TNF-α, our findings did not reveal differences between groups but an increase in the polyphenol and carrageenan group if compared to the carrageenan only group. As for antioxidant polyphenols' properties, no differences between groups in the serum glutathione were found. CONCLUSIONS: Altogether, this investigation shows that olivepolyphenols in the mouse may modulate the levels of cytokines having a role in the process of inflammation as TNF-α and IL-10.
Authors: Andreia F R Silva; Daniela Resende; Mariana Monteiro; Manuel A Coimbra; Artur M S Silva; Susana M Cardoso Journal: Antioxidants (Basel) Date: 2020-12-08
Authors: Andreia Nunes; Lídia Gonçalves; Joana Marto; Ana Margarida Martins; Alexandra N Silva; Pedro Pinto; Marta Martins; Carmo Fraga; Helena Margarida Ribeiro Journal: Pharmaceutics Date: 2021-03-30 Impact factor: 6.321
Authors: Flavio M Ceci; Mauro Ceccanti; Carla Petrella; Mario Vitali; Marisa P Messina; George N Chaldakov; Antonio Greco; Massimo Ralli; Marco Lucarelli; Antonio Angeloni; Marco Fiore; Giampiero Ferraguti Journal: Curr Neurovasc Res Date: 2021 Impact factor: 1.990