Nardhy Gomez-Lopez1,2,3, Roberto Romero1,4,5,6, Olesya Plazyo1,2, Bogdan Panaitescu7, Amy E Furcron1,2, Derek Miller1,2,3, Tamara Roumayah1, Emily Flom1, Sonia S Hassan1,2. 1. Perinatology Research Branch, Program for Perinatal Research and Obstetrics, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. 2. Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. 3. Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, USA. 4. Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA. 5. Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, USA. 6. Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA. 7. Department of Pediatrics, Neonatology Division, Wayne State University School of Medicine, Detroit, MI, USA.
Abstract
PROBLEM: Sterile intra-amniotic inflammation is associated with spontaneous preterm labor. Alarmins are proposed to mediate this inflammatory process. The aim of this study was to determine whether intra-amniotic administration of an alarmin, HMGB1, could induce preterm labor/birth. METHOD OF STUDY: Pregnant B6 mice were intra-amniotically or intraperitoneally injected with HMGB1 or PBS (control). Following injection, the gestational age and the rates of preterm birth and pup mortality were recorded. RESULTS: Intra-amniotic injection of HMGB1 led to preterm labor/birth [HMGB1 57% (4/7) versus PBS 0% (0/6); P = 0.049) and a high rate of pup mortality at week 1 [HMGB1 60.9 ± 11.7% (25/41) versus PBS 28.9 ± 12.6% (11/38); P = 0.001). Intraperitoneal injection of HMGB1 did not induce preterm labor/birth. CONCLUSION: Intra-amniotic administration of HMGB1 induces preterm labor/birth.
PROBLEM: Sterile intra-amniotic inflammation is associated with spontaneous preterm labor. Alarmins are proposed to mediate this inflammatory process. The aim of this study was to determine whether intra-amniotic administration of an alarmin, HMGB1, could induce preterm labor/birth. METHOD OF STUDY: Pregnant B6 mice were intra-amniotically or intraperitoneally injected with HMGB1 or PBS (control). Following injection, the gestational age and the rates of preterm birth and pup mortality were recorded. RESULTS:Intra-amniotic injection of HMGB1 led to preterm labor/birth [HMGB1 57% (4/7) versus PBS 0% (0/6); P = 0.049) and a high rate of pup mortality at week 1 [HMGB1 60.9 ± 11.7% (25/41) versus PBS 28.9 ± 12.6% (11/38); P = 0.001). Intraperitoneal injection of HMGB1 did not induce preterm labor/birth. CONCLUSION:Intra-amniotic administration of HMGB1 induces preterm labor/birth.
Authors: U Andersson; H Wang; K Palmblad; A C Aveberger; O Bloom; H Erlandsson-Harris; A Janson; R Kokkola; M Zhang; H Yang; K J Tracey Journal: J Exp Med Date: 2000-08-21 Impact factor: 14.307
Authors: Emily E Hadley; Samantha Sheller-Miller; George Saade; Carlos Salomon; Sam Mesiano; Robert N Taylor; Brandie D Taylor; Ramkumar Menon Journal: Am J Obstet Gynecol Date: 2018-08-21 Impact factor: 8.661
Authors: K Motomura; R Romero; V Garcia-Flores; Y Leng; Y Xu; J Galaz; R Slutsky; D Levenson; N Gomez-Lopez Journal: Mol Hum Reprod Date: 2020-09-01 Impact factor: 4.025