Philipp Pierdzioch1, Stefan Hartwig2, Sascha R Herbst1, Jan Dirk Raguse2, Henrik Dommisch3, Shady Abu-Sirhan1, Henrik C Wirtz1, Moritz Hertel4, Sebastian Paris1, Saskia Preissner5. 1. Department of Operative and Preventive Dentistry, Charité - Universitätsmedizin Berlin, Assmanshauser Str. 4-6, 14197, Berlin, Germany. 2. Department of Oral and Maxillofacial Surgery/Clinical Navigation, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany. 3. Department of Periodontology and Synoptic Dentistry, Charité - Universitätsmedizin Berlin, Assmanshauser Str. 4-6, 14197, Berlin, Germany. 4. Department of Oral Medicine, Dental Radiology and Oral Surgery, Charité - Universitätsmedizin Berlin, Assmanshauser Str. 4-6, 14197, Berlin, Germany. 5. Department of Operative and Preventive Dentistry, Charité - Universitätsmedizin Berlin, Assmanshauser Str. 4-6, 14197, Berlin, Germany. saskia.preissner@charite.de.
Abstract
OBJECTIVES: The aim of this study was to evaluate the antimicrobial effect of cold plasma (CP) on infected dentin surfaces in vitro and ex vivo. MATERIALS AND METHODS: To examine the effect of cold plasma on root surfaces, 24 root surfaces were infected with Streptococcus mitis. Specimens were randomly divided into three groups: Within the control group (C), root surfaces were rinsed with NaCl; root surfaces in the second group were additionally scaled and root planed (SRP), and in the third group, root surfaces were rinsed, scaled, root planed, and in addition, CP was applied (SRP + CP). To examine the effect of CP on root caries lesions (RCLs), 16 freshly extracted teeth with symmetrical carious lesions were equally divided into two groups. In the control group, carious lesions were treated with chlorhexidine (CHX), whereas CHX was applied in conjunction with CP in the test group (CHX + CP). For microbiological analysis, dentin samples were serially diluted and CFU counts were estimated after 24 h of incubation. RESULTS: Compared to C, mean CFU values for SRP and SRP + CP were significantly lower (p < 0.05). In addition, mean CFUs for SRP + CP were reduced to 0 and, therefore, significantly lower than SRP (2.98 log CFU/mL) alone (p = 0.000, Mann-Whitney U). Regarding RCLs, significantly lower mean CFU values were observed for CHX + CP when compared to CHX (4.45 vs. 2.67 log CFU/mL, p = 0.002, Mann-Whitney U test). CONCLUSIONS: For disinfection of exposed root surfaces, the adjunctive application of CP is promising. In addition, the combined application of CHX + CP has the potential to disinfect root dentin surfaces. CLINICAL RELEVANCE: It was shown that the combination of cold plasma with CHX is the best available option for the disinfection of root surfaces.
OBJECTIVES: The aim of this study was to evaluate the antimicrobial effect of cold plasma (CP) on infected dentin surfaces in vitro and ex vivo. MATERIALS AND METHODS: To examine the effect of cold plasma on root surfaces, 24 root surfaces were infected with Streptococcus mitis. Specimens were randomly divided into three groups: Within the control group (C), root surfaces were rinsed with NaCl; root surfaces in the second group were additionally scaled and root planed (SRP), and in the third group, root surfaces were rinsed, scaled, root planed, and in addition, CP was applied (SRP + CP). To examine the effect of CP on root caries lesions (RCLs), 16 freshly extracted teeth with symmetrical carious lesions were equally divided into two groups. In the control group, carious lesions were treated with chlorhexidine (CHX), whereas CHX was applied in conjunction with CP in the test group (CHX + CP). For microbiological analysis, dentin samples were serially diluted and CFU counts were estimated after 24 h of incubation. RESULTS: Compared to C, mean CFU values for SRP and SRP + CP were significantly lower (p < 0.05). In addition, mean CFUs for SRP + CP were reduced to 0 and, therefore, significantly lower than SRP (2.98 log CFU/mL) alone (p = 0.000, Mann-Whitney U). Regarding RCLs, significantly lower mean CFU values were observed for CHX + CP when compared to CHX (4.45 vs. 2.67 log CFU/mL, p = 0.002, Mann-Whitney U test). CONCLUSIONS: For disinfection of exposed root surfaces, the adjunctive application of CP is promising. In addition, the combined application of CHX + CP has the potential to disinfect root dentin surfaces. CLINICAL RELEVANCE: It was shown that the combination of cold plasma with CHX is the best available option for the disinfection of root surfaces.
Authors: Stefan Rupf; Antje Lehmann; Matthias Hannig; Barbara Schäfer; Andreas Schubert; Uwe Feldmann; Axel Schindler Journal: J Med Microbiol Date: 2009-11-12 Impact factor: 2.472
Authors: Rutger Matthes; Claudia Bender; Rabea Schlüter; Ina Koban; René Bussiahn; Stephan Reuter; Jürgen Lademann; Klaus-Dieter Weltmann; Axel Kramer Journal: PLoS One Date: 2013-07-24 Impact factor: 3.240