Giulia Di Pietro1, Silvia Capuani2, Guglielmo Manenti3, Vincenzo Vinicola4, Armando Fusco3, Jacopo Baldi5, Manuel Scimeca6, Gisela Hagberg7, Marco Bozzali8, Giovanni Simonetti3, Umberto Tarantino5. 1. Center for Life NanoScience@LaSapienza, Istituto Italiano di Tecnologia, Rome, Italy; CNR ISC UOS Roma Sapienza, Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy. 2. CNR ISC UOS Roma Sapienza, Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy; Enrico Fermi Center, Rome, Italy. Electronic address: silvia.capuani@roma1.infn.it. 3. Department of Diagnostic Imaging and Interventional Radiology, Molecular Imaging an Radiotherapy, PTV Foundation "Tor Vergata" University of Rome, Rome, Italy. 4. Center for the prevention, diagnosis and treatment of osteoporosis, Rehabilitation Hospital IRCCS Santa Lucia Foundation, Rome, Italy. 5. Department of Orthopaedics and Traumatology, PTV Foundation, "Tor Vergata" University of Rome, Rome, Italy. 6. Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy; "Multidisciplinary Study of the Effects of Microgravity on Bone Cells" Project, Italian Space Agency (ASI), Spatial Biomedicine Center, Via del Politecnico snc, Rome, Italy. 7. Department of Radiology, Biomedical Magnetic Resonance University Hospital Tübingen and High Field Magnetic Resonance, Max-Planck Institute for Biological Cybernetics Tübingen, Tübingen, Germany. 8. Neuroimaging Laboratory, Santa Lucia Foundation, Rome, Italy.
Abstract
RATIONALE AND OBJECTIVES: To characterize the lipidic profile of bone marrow in the calcaneus and femoral neck of healthy, osteopenic, and osteoporotic women, by using magnetic resonance spectroscopy (MRS) at 3T. The final goal was to identify specific metabolites with the potential ability to discriminate between healthy, osteopenic, and osteoporotic subjects. MATERIALS AND METHODS: Sixty-two and thirty three postmenopausal women recruited to investigate calcaneus and femoral neck, respectively, underwent a bone mineral density (BMD) measurement to be classified as healthy subjects (n = 22), osteopenic (n = 45), or osteoporotic (n = 28) patients. MRS spectra were used to quantify and compare bone marrow fat resonances between the three BMD groups. Between-group differences were tested using a Welch analysis of variance. Multiple comparisons were made with the Games-Howell correction. Relationships between pairs of variables were assessed with linear correlation analysis. Reproducibility analysis was performed for all the lipid resonances in both sites. RESULTS: The reproducibility was satisfactory. In femoral neck, methylene (L13), glycerol (L41, L43), and total lipid resonances were significantly lower in healthy as compared to osteoporotic subjects. On the other hand, in calcaneus, L13/glycerol significantly discriminated between osteopenic and osteoporotic subjects whereas L13/(unsaturated lipid) discriminated between healthy and osteopenic group. However, the reproducibility of both unsaturated lipid and glycerol resonances were less optimal. CONCLUSIONS: MRS of bone marrow lipid profiles from peripheral skeletal sites may be a promising tool for screening of large population to identify individuals with or at risk for developing osteoporosis. Moreover, it provides information about the metabolic changes occurring in bone marrow with the development of osteoporosis, which are skeletal site dependent.
RATIONALE AND OBJECTIVES: To characterize the lipidic profile of bone marrow in the calcaneus and femoral neck of healthy, osteopenic, and osteoporoticwomen, by using magnetic resonance spectroscopy (MRS) at 3T. The final goal was to identify specific metabolites with the potential ability to discriminate between healthy, osteopenic, and osteoporotic subjects. MATERIALS AND METHODS: Sixty-two and thirty three postmenopausal women recruited to investigate calcaneus and femoral neck, respectively, underwent a bone mineral density (BMD) measurement to be classified as healthy subjects (n = 22), osteopenic (n = 45), or osteoporotic (n = 28) patients. MRS spectra were used to quantify and compare bone marrow fat resonances between the three BMD groups. Between-group differences were tested using a Welch analysis of variance. Multiple comparisons were made with the Games-Howell correction. Relationships between pairs of variables were assessed with linear correlation analysis. Reproducibility analysis was performed for all the lipid resonances in both sites. RESULTS: The reproducibility was satisfactory. In femoral neck, methylene (L13), glycerol (L41, L43), and total lipid resonances were significantly lower in healthy as compared to osteoporotic subjects. On the other hand, in calcaneus, L13/glycerol significantly discriminated between osteopenic and osteoporotic subjects whereas L13/(unsaturated lipid) discriminated between healthy and osteopenic group. However, the reproducibility of both unsaturated lipid and glycerol resonances were less optimal. CONCLUSIONS: MRS of bone marrow lipid profiles from peripheral skeletal sites may be a promising tool for screening of large population to identify individuals with or at risk for developing osteoporosis. Moreover, it provides information about the metabolic changes occurring in bone marrow with the development of osteoporosis, which are skeletal site dependent.
Authors: Sridhar Vajapeyam; Kirsten Ecklund; Robert V Mulkern; Henry A Feldman; Jennifer M O'Donnell; Amy D DiVasta; Clifford J Rosen; Catherine M Gordon Journal: Bone Date: 2018-02-26 Impact factor: 4.398
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