Henrique Melo Natalin1, Arthur Feierabend Engracia Garcia1, Leandra Naira Zambeli Ramalho2, Carolina Baraldi Araujo Restini3. 1. Faculty of Medicine, University of Ribeirão Preto (UNAERP), Medicine School Ribeirão Preto, SP, Brazil. 2. Department of Cellular and Molecular Pathology, School of Medicine of Ribeirão Preto, University of São Paulo, SP, Brasil. 3. Faculty of Medicine, University of Ribeirão Preto (UNAERP), Medicine School Ribeirão Preto, SP, Brazil. Electronic address: carolbaraldi@hotmail.com.
Abstract
BACKGROUND: Renin-angiotensin system triggers vascular remodeling and fibrosis during the renovascular hypertensive two-kidney, one-clip (2K1C) model by oxidative-stress-mediated mechanisms. Thus, we hypothesized that the chronic treatment with the polyphenolic antioxidant resveratrol would improve the vasoprotective effects promoted by the chronic treatment with the angiotensin-converting enzyme inhibitor (ACEi) captopril in 2K1C hypertensive rats. Our main objective was to evaluate the effects of the combined treatment with resveratrol and captopril on vascular remodeling and fibrosis in 2K1C rats. METHODS: Male Wistar rats underwent to unilateral renal stenosis by 2K1C Goldblatt model. Six weeks after surgery, rat systolic blood pressure (SBP) was measured by indirect tail-cuff plethysmography. 2K1C rats were considered hypertensive when presenting SBP higher than 160 mmHg and underwent resveratrol (20 mg/kg), captopril (6 or 12 mg/kg), or resveratrol (20 mg/kg) combined with captopril (6 or 12 mg/kg) treatment for 3 weeks. Nine weeks after surgery, rat SBP was measured, and rat thoracic aorta was isolated for histological assays with hematoxylin/eosin or Picrosirius Red to evaluate aortic remodeling and fibrosis, respectively. RESULTS: Oral treatment of 2K1C hypertensive rats with resveratrol (20 mg/kg) combined with the dose-dependent ACEi captopril (6 and 12 mg/kg) resulted in lesser aortic thickening and reduced aortic fibrosis. Resveratrol (20 mg/kg) promoted a more expressive hypotensive effect with captopril (12 mg/kg) in 2K1C rats than the treatment with isolated captopril (12 mg/kg). CONCLUSION: Resveratrol improves the vasoprotective effects promoted by captopril on aortic remodeling and fibrosis during renovascular hypertension probably by synergic mechanisms involving antioxidant actions and nitric oxide generation.
BACKGROUND: Renin-angiotensin system triggers vascular remodeling and fibrosis during the renovascular hypertensive two-kidney, one-clip (2K1C) model by oxidative-stress-mediated mechanisms. Thus, we hypothesized that the chronic treatment with the polyphenolic antioxidant resveratrol would improve the vasoprotective effects promoted by the chronic treatment with the angiotensin-converting enzyme inhibitor (ACEi) captopril in 2K1C hypertensiverats. Our main objective was to evaluate the effects of the combined treatment with resveratrol and captopril on vascular remodeling and fibrosis in 2K1C rats. METHODS: Male Wistar rats underwent to unilateral renal stenosis by 2K1C Goldblatt model. Six weeks after surgery, rat systolic blood pressure (SBP) was measured by indirect tail-cuff plethysmography. 2K1C rats were considered hypertensive when presenting SBP higher than 160 mmHg and underwent resveratrol (20 mg/kg), captopril (6 or 12 mg/kg), or resveratrol (20 mg/kg) combined with captopril (6 or 12 mg/kg) treatment for 3 weeks. Nine weeks after surgery, rat SBP was measured, and rat thoracic aorta was isolated for histological assays with hematoxylin/eosin or Picrosirius Red to evaluate aortic remodeling and fibrosis, respectively. RESULTS: Oral treatment of 2K1C hypertensiverats with resveratrol (20 mg/kg) combined with the dose-dependent ACEi captopril (6 and 12 mg/kg) resulted in lesser aortic thickening and reduced aortic fibrosis. Resveratrol (20 mg/kg) promoted a more expressive hypotensive effect with captopril (12 mg/kg) in 2K1C rats than the treatment with isolated captopril (12 mg/kg). CONCLUSION:Resveratrol improves the vasoprotective effects promoted by captopril on aortic remodeling and fibrosis during renovascular hypertension probably by synergic mechanisms involving antioxidant actions and nitric oxide generation.
Authors: Sin Young Choi; Zhe Hao Piao; Li Jin; Jung Ha Kim; Gwi Ran Kim; Yuhee Ryu; Ming Quan Lin; Hyung-Seok Kim; Hae Jin Kee; Myung Ho Jeong Journal: PLoS One Date: 2016-11-30 Impact factor: 3.240
Authors: Juan M Suárez-Rivero; Carmen J Pastor-Maldonado; Ana Romero-González; David Gómez-Fernandez; Suleva Povea-Cabello; Mónica Álvarez-Córdoba; Irene Villalón-García; Marta Talaverón-Rey; Alejandra Suárez-Carrillo; Manuel Munuera-Cabeza; José A Sánchez-Alcázar Journal: Front Pharmacol Date: 2022-03-18 Impact factor: 5.810
Authors: Carolina B A Restini; Arthur F E Garcia; Henrique M Natalin; Mariane F A Carmo; Vinicius F Nowicki; Elen Rizzi; Leandra N Z Ramalho Journal: Yale J Biol Med Date: 2022-03-31