Literature DB >> 2675845

Discriminator base of tRNA(Asp) is involved in amino acid acceptor activity.

T Hasegawa1, H Himeno, H Ishikura, M Shimizu.   

Abstract

Unmodified E. coli tRNA(Asp) was obtained by in vitro transcription with T7 RNA polymerase from a plasmid carrying a tRNA(Asp) sequence adjacent to the T7 promoter. The transcript exhibited almost the same level of amino acid acceptor activity as intact tRNA(Asp), and the kinetic parameters were also similar. However, substitution of the discriminator base (the fourth base from the 3'-end) markedly affected the amino acid acceptor activity. These results suggest that the discriminator base in tRNA(Asp) plays an important role in the recognition of aspartyl-tRNA synthetase.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2675845     DOI： 10.1016/0006-291x(89)91154-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  15 in total

1.  Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo.

Authors:  Franck Martin; Sharief Barends; Gilbert Eriani
Journal:  Nucleic Acids Res       Date:  2004-08-02       Impact factor: 16.971

2.  Identity determinants of E. coli tryptophan tRNA.

Authors:  H Himeno; T Hasegawa; H Asahara; K Tamura; M Shimizu
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

3.  Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.

Authors:  H Himeno; T Hasegawa; T Ueda; K Watanabe; M Shimizu
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

4.  tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition.

Authors:  S T Nonekowski; G A Garcia
Journal:  RNA       Date:  2001-10       Impact factor: 4.942

5.  3' truncated tRNAArg is essential for in vitro specific cleavage of partially synthesized mouse 18S rRNA.

Authors:  M Nashimoto
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

6.  Nucleotides that determine Escherichia coli tRNA(Arg) and tRNA(Lys) acceptor identities revealed by analyses of mutant opal and amber suppressor tRNAs.

Authors:  W H McClain; K Foss; R A Jenkins; J Schneider
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

7.  Involvement of the size and sequence of the anticodon loop in tRNA recognition by mammalian and E. coli methionyl-tRNA synthetases.

Authors:  T Meinnel; Y Mechulam; G Fayat; S Blanquet
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

8.  In vitro study of E.coli tRNA(Arg) and tRNA(Lys) identity elements.

Authors:  K Tamura; H Himeno; H Asahara; T Hasegawa; M Shimizu
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

Review 9.  Experimental studies on the origin of the genetic code and the process of protein synthesis: a review update.

Authors:  J C Lacey; N S Wickramasinghe; G W Cook
Journal:  Orig Life Evol Biosph       Date:  1992       Impact factor: 1.950

10.  The role of anticodon bases and the discriminator nucleotide in the recognition of some E. coli tRNAs by their aminoacyl-tRNA synthetases.

Authors:  M Shimizu; H Asahara; K Tamura; T Hasegawa; H Himeno
Journal:  J Mol Evol       Date:  1992-11       Impact factor: 2.395
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.