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Literature DB >> 26756539

Solid-State NMR of PEGylated Proteins.

Enrico Ravera¹, Silvia Ciambellotti¹, Linda Cerofolini¹, Tommaso Martelli², Tatiana Kozyreva², Caterina Bernacchioni¹, Stefano Giuntini¹, Marco Fragai¹, Paola Turano¹, Claudio Luchinat^3,4.

Abstract

PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic-level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid-state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use.

Entities: Chemical

Keywords: PEGylated proteins; biopharmaceuticals; protein modification; protein structures; solid-state NMR

Mesh：

Substances：

Year: 2016 PMID： 26756539 DOI： 10.1002/anie.201510148

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

12 in total

1. Variability and conservation of structural domains in divide-and-conquer approaches.

Authors: Thomas Wiegand; Carole Gardiennet; Riccardo Cadalbert; Denis Lacabanne; Britta Kunert; Laurent Terradot; Anja Böckmann; Beat H Meier
Journal: J Biomol NMR Date: 2016-05-30 Impact factor: 2.835

2. Chemistry at the protein-mineral interface in L-ferritin assists the assembly of a functional (μ³-oxo)Tris[(μ²-peroxo)] triiron(III) cluster.

Authors: Cecilia Pozzi; Silvia Ciambellotti; Caterina Bernacchioni; Flavio Di Pisa; Stefano Mangani; Paola Turano
Journal: Proc Natl Acad Sci U S A Date: 2017-02-15 Impact factor: 11.205

3. Atomic-Scale View of Protein-PEG Interactions that Redirect the Thermal Unfolding Pathway of PEGylated Human Galectin-3.

Authors: Amanda Pritzlaff; Guillaume Ferré; Emma Mulry; Ling Lin; Niloofar Gopal Pour; Daniel A Savin; Michael E Harris; Matthew T Eddy
Journal: Angew Chem Int Ed Engl Date: 2022-08-25 Impact factor: 16.823

4. Atomic structural details of a protein grafted onto gold nanoparticles.

Authors: Stefano Giuntini; Linda Cerofolini; Enrico Ravera; Marco Fragai; Claudio Luchinat
Journal: Sci Rep Date: 2017-12-20 Impact factor: 4.379

Review 5. Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques.

Authors: Linda Cerofolini; Marco Fragai; Enrico Ravera; Christoph A Diebolder; Ludovic Renault; Vito Calderone
Journal: Biomolecules Date: 2019-08-14

10. A protocol to automatically calculate homo-oligomeric protein structures through the integration of evolutionary constraints and NMR ambiguous contacts.

Authors: Davide Sala; Linda Cerofolini; Marco Fragai; Andrea Giachetti; Claudio Luchinat; Antonio Rosato
Journal: Comput Struct Biotechnol J Date: 2019-12-26 Impact factor: 7.271

Solid-State NMR of PEGylated Proteins.

1. Variability and conservation of structural domains in divide-and-conquer approaches.

2. Chemistry at the protein-mineral interface in L-ferritin assists the assembly of a functional (μ³-oxo)Tris[(μ²-peroxo)] triiron(III) cluster.

3. Atomic-Scale View of Protein-PEG Interactions that Redirect the Thermal Unfolding Pathway of PEGylated Human Galectin-3.

4. Atomic structural details of a protein grafted onto gold nanoparticles.

Review 5. Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques.

6. L-Ferritin targets breast cancer stem cells and delivers therapeutic and imaging agents.

7. Characterization of the Conjugation Pattern in Large Polysaccharide-Protein Conjugates by NMR Spectroscopy.

8. Cancer cell death induced by ferritins and the peculiar role of their labile iron pool.

9. Sedimentation Yields Long-Term Stable Protein Samples as Shown by Solid-State NMR.

10. A protocol to automatically calculate homo-oligomeric protein structures through the integration of evolutionary constraints and NMR ambiguous contacts.