Lang Qin1, Wenming Xu2, Xiaohong Li3, Wentong Meng4, Lijuan Hu5, Zhijuan Luo6, Yan Wang7, Shan Luo8, Shangwei Li9. 1. The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, China. Electronic address: cacier@126.com. 2. SCU-CUHK Joint Laboratory for Reproductive Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address: xuwenming1973@163.com. 3. The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, China. Electronic address: lixiaohongham_001@163.com. 4. Laboratory of Stem Cell Biology and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu City, Sichuan Province 610041, China. Electronic address: mwentong@sina.com. 5. Department of Immunology, College of Pre-clinical and Forensic Medicine, Sichuan University, Chengdu City, Sichuan Province, 610041, China. Electronic address: hulijuan7572@163.com. 6. Department of Immunology, College of Pre-clinical and Forensic Medicine, Sichuan University, Chengdu City, Sichuan Province, 610041, China. Electronic address: juanserre@gmail.com. 7. The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, China. Electronic address: wangyy1210@163.com. 8. The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, China. Electronic address: luo_shan76@yahoo.com.cn. 9. The Reproductive Centre, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, China. Electronic address: cacier@163.com.
Abstract
OBJECTIVE: Immune dysregulation may play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). The purpose of this study was to investigate the Th1 and Th2-related cytokine profile in local ovary of women with PCOS. STUDY DESIGN: The T lymphocytes of follicular fluid (FF) were obtained at the time of oocyte retrieval before in-vitro fertilization (IVF) in woman with or without PCOS. After culturing with PMA, Ionomycin and Golgi stop agent, cells were detected for the intracellular cytokine production by flow cytometry. The profile of Th1 (IFN-γ, IL-2) and Th2 (IL-4, IL-10) cytokines of CD3(+) CD4(+)T lymphocyte subsets were analyzed through invert gating. These cytokines in FF were also evaluated by ELISA. RESULTS: Flow cytometry analysis showed that the production of Th1 (IFN-γ, IL-2) cytokines in FF lymphocytes in PCOS patients were significantly higher than those in controls; ELISA result also demonstrated that the concentration of Th1 cytokines (IFN-γ, IL-2) in FF in PCOS patients is significantly increased compared with those in controls. CONCLUSION: It is concluded that the immune dominance of Th1 may be the immunological feature of the ovary in PCOS patients. It might participate in the immune pathogenesis in the ovary of PCOS patients. These results suggest that chronic inflammation maybe one of the underlying mechanism for the pathogenesis of PCOS.
OBJECTIVE: Immune dysregulation may play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). The purpose of this study was to investigate the Th1 and Th2-related cytokine profile in local ovary of women with PCOS. STUDY DESIGN: The T lymphocytes of follicular fluid (FF) were obtained at the time of oocyte retrieval before in-vitro fertilization (IVF) in woman with or without PCOS. After culturing with PMA, Ionomycin and Golgi stop agent, cells were detected for the intracellular cytokine production by flow cytometry. The profile of Th1 (IFN-γ, IL-2) and Th2 (IL-4, IL-10) cytokines of CD3(+) CD4(+)T lymphocyte subsets were analyzed through invert gating. These cytokines in FF were also evaluated by ELISA. RESULTS: Flow cytometry analysis showed that the production of Th1 (IFN-γ, IL-2) cytokines in FF lymphocytes in PCOSpatients were significantly higher than those in controls; ELISA result also demonstrated that the concentration of Th1 cytokines (IFN-γ, IL-2) in FF in PCOSpatients is significantly increased compared with those in controls. CONCLUSION: It is concluded that the immune dominance of Th1 may be the immunological feature of the ovary in PCOSpatients. It might participate in the immune pathogenesis in the ovary of PCOSpatients. These results suggest that chronic inflammation maybe one of the underlying mechanism for the pathogenesis of PCOS.
Authors: Cecily V Bishop; Taylor E Reiter; David W Erikson; Carol B Hanna; Brittany L Daughtry; Shawn L Chavez; Jon D Hennebold; Richard L Stouffer Journal: J Assist Reprod Genet Date: 2019-06-11 Impact factor: 3.412