| Literature DB >> 26749588 |
Jianhong Zuo1,2, Juan Wen3, Mingsheng Lei4, Meiling Wen5, Sai Li3,5, Xiu Lv3,5, Zhaoyang Luo6, Gebo Wen7.
Abstract
The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node metastases (P < 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P < 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.Entities:
Keywords: E-cadherin; Epithelial–mesenchymal transition; Glucose transporter-1; Laryngeal carcinoma; Vimentin
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Year: 2016 PMID： 26749588 DOI： 10.1007/s12032-015-0716-6
Source DB: PubMed Journal: Med Oncol ISSN： 1357-0560 Impact factor: 3.064