| Literature DB >> 26736268 |
G Adriani, D Ma, A Pavesi, E L K Goh, R D Kamm.
Abstract
The need for a blood-brain barrier (BBB) model that accurately mimics the physiological characteristics of the in-vivo situation is well-recognized by researchers in academia and industry. However, there is currently no in-vitro model allowing studies of neuronal growth and/or function influenced by factors from the blood that cross through the BBB. Therefore, we established a 3D triple co-culture microfluidic system using human umbilical vein endothelial cells (HUVEC) together with primary rat astrocytes and neurons. Immunostaining confirmed the successful triple co-culture system consisting of an intact BBB with tight intercellular junctions in the endothelial monolayer. The BBB selective permeability was determined by a fluorescent-based assay using dextrans of different molecular weights. Finally, neuron functionality was demonstrated by calcium imaging.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26736268 DOI： 10.1109/EMBC.2015.7318368
Source DB: PubMed Journal: Conf Proc IEEE Eng Med Biol Soc ISSN： 1557-170X