M Cenk Durmuşlar1, Umut Ballı2, Figen Öngöz Dede2, Şeyma Bozkurt Doğan2, A Ferhat Mısır3, Emre Barış4, Zehra Yılmaz5, H Hamdi Çelik6, Alper Vatansever6. 1. Bülent Ecevit University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey. Electronic address: cenkdurmuslar@hotmail.com. 2. Bülent Ecevit University Faculty of Dentistry, Department of Periodontology, Zonguldak, Turkey. 3. Bülent Ecevit University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey. 4. Gazi University Faculty of Dentistry, Department of Oral Pathology, Ankara, Turkey. 5. Harran University Faculty of Medicine, Department of Pharmacology, Turkey. 6. Hacettepe University Faculty of Medicine, Department of Anatomy, Turkey.
Abstract
OBJECTIVES: This study aimed to investigate the effect of platelet-rich fibrin on bone regeneration in critical size defects in the calvaria of diabetic rabbits. STUDY DESIGN: In total, 40 male New Zealand rabbits, were divided into two groups a non-diabetic control group (Group A) and a diabetic experimental group (Group B). Two bicortical circular defects 15 mm in diameter were created in the parietal bone of each animal. Each group was further divided into four groups: subgroup E, the defect was left empty; subgroup PRF, the defects were filled only with PRF; subgroup AB, the defects were filled with autogenous bone; subgroup AB + PRF, the defects were filled with autogenous bone combined with PRF. The animals sacrificed at 4 weeks and 8 weeks. Bone formation was assessed by micro-computed tomography (micro-CT) scanning, histological and histomorphometric analysis. RESULT: The total percent of new bone was the lowest in group A-E (6.77 ± 0.21 at 4 weeks, 11.01 ± 0.37 at 8 weeks) and highest in group A-AB + PRF (21.66 ± 0.91 at 4 weeks, 37.46 ± 1.25 at 8 weeks; p < 0.05). The mean percent of new bone was greatest in group B-AB + PRF at 4 and 8 weeks (16.87 ± 0.92, 29.59 ± 1.09, respectively) and lowest in group B-E (5.83 ± 0.09 at 4 weeks, 7.36 ± 1.02 at 8 weeks). CONCLUSION: This study, despite its limitations, showed that PRF can be used safely and that PRF induced bone healing in diabetic rabbits.
OBJECTIVES: This study aimed to investigate the effect of platelet-rich fibrin on bone regeneration in critical size defects in the calvaria of diabetic rabbits. STUDY DESIGN: In total, 40 male New Zealand rabbits, were divided into two groups a non-diabetic control group (Group A) and a diabetic experimental group (Group B). Two bicortical circular defects 15 mm in diameter were created in the parietal bone of each animal. Each group was further divided into four groups: subgroup E, the defect was left empty; subgroup PRF, the defects were filled only with PRF; subgroup AB, the defects were filled with autogenous bone; subgroup AB + PRF, the defects were filled with autogenous bone combined with PRF. The animals sacrificed at 4 weeks and 8 weeks. Bone formation was assessed by micro-computed tomography (micro-CT) scanning, histological and histomorphometric analysis. RESULT: The total percent of new bone was the lowest in group A-E (6.77 ± 0.21 at 4 weeks, 11.01 ± 0.37 at 8 weeks) and highest in group A-AB + PRF (21.66 ± 0.91 at 4 weeks, 37.46 ± 1.25 at 8 weeks; p < 0.05). The mean percent of new bone was greatest in group B-AB + PRF at 4 and 8 weeks (16.87 ± 0.92, 29.59 ± 1.09, respectively) and lowest in group B-E (5.83 ± 0.09 at 4 weeks, 7.36 ± 1.02 at 8 weeks). CONCLUSION: This study, despite its limitations, showed that PRF can be used safely and that PRF induced bone healing in diabetic rabbits.
Authors: Behnoush Khorsand; Nate Nicholson; Anh-Vu Do; John E Femino; James A Martin; Emily Petersen; Brian Guetschow; Douglas C Fredericks; Aliasger K Salem Journal: J Control Release Date: 2017-01-07 Impact factor: 11.467
Authors: Michala Rampichová; Matej Buzgo; Andrea Míčková; Karolína Vocetková; Věra Sovková; Věra Lukášová; Eva Filová; Franco Rustichelli; Evžen Amler Journal: Int J Nanomedicine Date: 2017-01-06