Estibaliz Alegre1, Leyre Zubiri2, Jose Luis Perez-Gracia2, María González-Cao3, Lourdes Soria2, Salvador Martín-Algarra2, Alvaro González4. 1. Laboratory of Biochemistry, University Clinic of Navarra, Spain. 2. Department of Medical Oncology, University Clinic of Navarra, Spain. 3. Translational Cancer Research Unit, Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital, Barcelona, Spain. 4. Laboratory of Biochemistry, University Clinic of Navarra, Spain. Electronic address: agonzaleh@unav.es.
Abstract
BACKGROUND: Malignant melanoma is an aggressive cancer with an increasing incidence. Exosomes are actively secreted microvesicles, whose characteristics reflect those of the cell they are originated in. The aim of this study was to identify and evaluate the presence of the melanoma biomarkers MIA, S100B and tyrosinase-related protein 2 (TYRP2) in exosomes and their potential clinical utility. METHODS: Serum samples were obtained from stage IV melanoma patients, melanoma-free patients and healthy controls. Exosomes were precipitated and TYRP2, MIA and S100B concentrations were quantified in serum, exosomes, and exosome-free serum. RESULTS: Both MIA and S100B were detected in exosomes and correlated significantly with serum concentrations (S100B: r=0.968; MIA: r=0.799; p<0.001). MIA and S100B concentrations in exosomes were significantly higher in melanoma patients than in healthy controls and disease-free patients. However, TYRP2 concentrations in exosomes did not differ between these three groups. ROC curves analysis rendered AUCs for MIA of 0.883 (p<0.01) and of 0.840 for S100B (p<0.01). Patients with exosome MIA concentration higher than 2.5 μg/L showed shorter median survival related to those with lower level (4 versus 11 months; p<0.05). CONCLUSIONS: MIA and S100B can be detected in exosomes from melanoma patients and their quantification presents diagnostic and prognostic utility.
BACKGROUND:Malignant melanoma is an aggressive cancer with an increasing incidence. Exosomes are actively secreted microvesicles, whose characteristics reflect those of the cell they are originated in. The aim of this study was to identify and evaluate the presence of the melanoma biomarkers MIA, S100B and tyrosinase-related protein 2 (TYRP2) in exosomes and their potential clinical utility. METHODS: Serum samples were obtained from stage IV melanomapatients, melanoma-freepatients and healthy controls. Exosomes were precipitated and TYRP2, MIA and S100B concentrations were quantified in serum, exosomes, and exosome-free serum. RESULTS: Both MIA and S100B were detected in exosomes and correlated significantly with serum concentrations (S100B: r=0.968; MIA: r=0.799; p<0.001). MIA and S100B concentrations in exosomes were significantly higher in melanomapatients than in healthy controls and disease-free patients. However, TYRP2 concentrations in exosomes did not differ between these three groups. ROC curves analysis rendered AUCs for MIA of 0.883 (p<0.01) and of 0.840 for S100B (p<0.01). Patients with exosome MIA concentration higher than 2.5 μg/L showed shorter median survival related to those with lower level (4 versus 11 months; p<0.05). CONCLUSIONS: MIA and S100B can be detected in exosomes from melanomapatients and their quantification presents diagnostic and prognostic utility.
Authors: Shin La Shu; Cheryl L Allen; Shawna Benjamin-Davalos; Marina Koroleva; Don MacFarland; Hans Minderman; Marc S Ernstoff Journal: Methods Mol Biol Date: 2021
Authors: Letizia Porcelli; Michele Guida; Simona Serratì; Amalia Azzariti; Simona De Summa; Roberta Di Fonte; Ivana De Risi; Marianna Garofoli; Mariapia Caputo; Antonio Negri; Sabino Strippoli Journal: J Immunother Cancer Date: 2021-05 Impact factor: 13.751