| Literature DB >> 26724083 |
M S DeVore1, D G Stich1, A M Keller1, C Cleyrat2, M E Phipps1, J A Hollingsworth1, D S Lidke2, B S Wilson2, P M Goodwin1, J H Werner1.
Abstract
We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.Mesh：
Year: 2015 PMID： 26724083 PMCID： PMC4676784 DOI： 10.1063/1.4937477
Source DB: PubMed Journal: Rev Sci Instrum ISSN： 0034-6748 Impact factor: 1.523