Fabiano B Carvalho1, Aline A Boligon2, Margareth L Athayde2, Maribel A Rubin1, Juliano Ferreira3, Gabriela Trevisan4. 1. Programa de Pós-graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil. 2. Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil. 3. Programa de Pós-graduação em Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina (UFSC), 88049-900 Florianópolis, SC, Brazil. 4. Programa de Pós-graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense (Unesc), 88806-000 Criciúma, SC, Brazil. Electronic address: gabitrev@hotmail.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Scutia buxifolia is a tree native to South America and is used as a cardiotonic agent; however, this property has not been associated with a clear mechanism or a specific compound. AIM OF THE STUDY: Given the importance of Na(+),K(+)-ATPase as a drug target in the treatment of heart failure, this study aimed to investigate the possible inhibitory effect of S. buxifolia crude extract and fractions (dichloromethane, ethyl acetate, and butanolic fractions), and identified compounds with effects on the activity of Na(+),K(+)-ATPase in vitro. MATERIALS AND METHODS: First, we characterized the crude extract and fractions by high-performance liquid chromatography, and then monitored their effects on the activity of Na(+),K(+)-ATPase obtained from heart muscle and brain membranes of adult male Wistar rats. RESULTS: We identified gallic acid, chlorogenic acid, caffeic acid, rutin, quercitrin, quercetin, and ursolic acid in S. buxifolia stem bark and leaves; quercitrin and ursolic acid were the main compounds in the ethyl acetate and dichloromethane fractions from leaves and stem bark. The crude extract (3 and 30mg/ml), and the ethyl acetate and dichloromethane fractions (0.1 and 1mg/ml) of both the stem bark and leaves inhibited Na(+),K(+)-ATPase activity in heart and brain samples. We found that, of the identified compounds, only ursolic acid (0.1mg/ml) was able to diminish Na(+), K(+)-ATPase activity in heart and brain samples. CONCLUSIONS: These data indicated that the cardiotonic effects of S. buxifolia may be due to the inhibition of Na(+),K(+)-ATPase activity in heart muscle, supporting the popular use of this plant as a treatment for heart failure.
ETHNOPHARMACOLOGICAL RELEVANCE: Scutia buxifolia is a tree native to South America and is used as a cardiotonic agent; however, this property has not been associated with a clear mechanism or a specific compound. AIM OF THE STUDY: Given the importance of Na(+),K(+)-ATPase as a drug target in the treatment of heart failure, this study aimed to investigate the possible inhibitory effect of S. buxifolia crude extract and fractions (dichloromethane, ethyl acetate, and butanolic fractions), and identified compounds with effects on the activity of Na(+),K(+)-ATPase in vitro. MATERIALS AND METHODS: First, we characterized the crude extract and fractions by high-performance liquid chromatography, and then monitored their effects on the activity of Na(+),K(+)-ATPase obtained from heart muscle and brain membranes of adult male Wistar rats. RESULTS: We identified gallic acid, chlorogenic acid, caffeic acid, rutin, quercitrin, quercetin, and ursolic acid in S. buxifolia stem bark and leaves; quercitrin and ursolic acid were the main compounds in the ethyl acetate and dichloromethane fractions from leaves and stem bark. The crude extract (3 and 30mg/ml), and the ethyl acetate and dichloromethane fractions (0.1 and 1mg/ml) of both the stem bark and leaves inhibited Na(+),K(+)-ATPase activity in heart and brain samples. We found that, of the identified compounds, only ursolic acid (0.1mg/ml) was able to diminish Na(+), K(+)-ATPase activity in heart and brain samples. CONCLUSIONS: These data indicated that the cardiotonic effects of S. buxifolia may be due to the inhibition of Na(+),K(+)-ATPase activity in heart muscle, supporting the popular use of this plant as a treatment for heart failure.
Authors: Fabiano B Carvalho; Jessié M Gutierres; Andressa Bueno; Paula Agostinho; Adriana M Zago; Juliano Vieira; Pâmela Frühauf; José L Cechella; Cristina Wayne Nogueira; Sara M Oliveira; Caroline Rizzi; Roselia M Spanevello; Marta M F Duarte; Thiago Duarte; Odir A Dellagostin; Cinthia M Andrade Journal: Mol Neurobiol Date: 2016-05-11 Impact factor: 5.590
Authors: Basiru O Ajiboye; Oluwafemi A Ojo; Marry A Okesola; Ayodele J Akinyemi; Justina Y Talabi; Olajumoke T Idowu; Adewale O Fadaka; Aline A Boligon; Marli M Anraku de Campos Journal: Food Sci Nutr Date: 2018-08-13 Impact factor: 2.863