AIM: Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants. MATERIAL AND METHODS: Titanium implants were placed in over-sized murine oral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri-implant bone. After fibrous encapsulation was established peri-implant injections of a liposomal formulation of WNT3A protein (L-WNT3A) or liposomal PBS (L-PBS) were then initiated. Quantitative assays were employed to analyse the effects of L-WNT3A treatment. RESULTS: Implants in gap-type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L-WNT3A or L-PBS injections were initiated. L-WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri-implant environment, cell proliferation and osteogenic protein expression. The amount of peri-implant bone and bone in contact with the implant were significantly higher in L-WNT3A cases. CONCLUSIONS: These data demonstrate L-WNT3A can induce peri-implant bone formation even in cases where fibrous encapsulation predominates.
AIM: Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants. MATERIAL AND METHODS:Titanium implants were placed in over-sized murineoral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri-implant bone. After fibrous encapsulation was established peri-implant injections of a liposomal formulation of WNT3A protein (L-WNT3A) or liposomal PBS (L-PBS) were then initiated. Quantitative assays were employed to analyse the effects of L-WNT3A treatment. RESULTS: Implants in gap-type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L-WNT3A or L-PBS injections were initiated. L-WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri-implant environment, cell proliferation and osteogenic protein expression. The amount of peri-implant bone and bone in contact with the implant were significantly higher in L-WNT3A cases. CONCLUSIONS: These data demonstrate L-WNT3A can induce peri-implant bone formation even in cases where fibrous encapsulation predominates.
Authors: Steven Minear; Philipp Leucht; Jie Jiang; Bo Liu; Arial Zeng; Christophe Fuerer; Roel Nusse; Jill A Helms Journal: Sci Transl Med Date: 2010-04-28 Impact factor: 17.956
Authors: X Yin; J Li; B Salmon; L Huang; W H Lim; B Liu; D J Hunter; R C Ransom; G Singh; M Gillette; S Zou; J A Helms Journal: J Dent Res Date: 2015-08-18 Impact factor: 6.116
Authors: Sylvain Mouraret; Daniel J Hunter; Claire Bardet; Antoine Popelut; John B Brunski; Catherine Chaussain; Philippe Bouchard; Jill A Helms Journal: J Clin Periodontol Date: 2013-12-08 Impact factor: 8.728
Authors: Nathan T Morrell; Philipp Leucht; Ludan Zhao; Jae-Beom Kim; Derk ten Berge; Karthik Ponnusamy; A Lyonel Carre; Henryk Dudek; Marie Zachlederova; Michael McElhaney; Shirley Brunton; Janet Gunzner; Marinella Callow; Paul Polakis; Mike Costa; Xiaoyan M Zhang; Jill A Helms; Roel Nusse Journal: PLoS One Date: 2008-08-13 Impact factor: 3.240
Authors: Benjamin R Coyac; Brian Leahy; Zhijun Li; Giuseppe Salvi; Xing Yin; John B Brunski; Jill A Helms Journal: Clin Oral Implants Res Date: 2020-09-14 Impact factor: 5.977
Authors: X Yuan; X Pei; Y Zhao; Z Li; C H Chen; U S Tulu; B Liu; L A Van Brunt; J B Brunski; J A Helms Journal: J Dent Res Date: 2018-04-02 Impact factor: 6.116
Authors: Zhijun Li; Masaki Arioka; Yindong Liu; Maziar Aghvami; Serdar Tulu; John B Brunski; Jill A Helms Journal: Bone Joint Res Date: 2020-05-16 Impact factor: 5.853
Authors: Sándor Farkasdi; Dávid Pammer; Róbert Rácz; Gergely Hriczó-Koperdák; Bence Tamás Szabó; Csaba Dobó-Nagy; Beáta Kerémi; József Blazsek; Frederic Cuisinier; Gang Wu; Gábor Varga Journal: Clin Oral Investig Date: 2018-10-29 Impact factor: 3.573