| Literature DB >> 26712706 |
Sasivimon Pramual1, Apinya Assavanig1, Magnus Bergkvist2, Carl A Batt3, Panya Sunintaboon4, Kriengsak Lirdprapamongkol5, Jisnuson Svasti5,6, Nuttawee Niamsiri7.
Abstract
In this study, we developed and investigated nanoparticles of biologically-derived, biodegradable polyhydroxyalkanoates (PHAs) as carriers of a hydrophobic photosensitizer, 5,10,15,20-Tetrakis(4-hydroxy-phenyl)-21H, 23H-porphine (pTHPP) for photodynamic therapy (PDT). Three PHA variants; polyhydroxybutyrate, poly(hydroxybutyrate-co-hydroxyvalerate) or P(HB-HV) with 12 and 50% HV were used to formulate pTHPP-loaded PHA nanoparticles by an emulsification-diffusion method, where we compared two different poly(vinyl alcohol) (PVA) stabilizers. The nanoparticles exhibited nano-scale spherical morphology under TEM and hydrodynamic diameters ranging from 169.0 to 211.2 nm with narrow size distribution. The amount of drug loaded and the drug entrapment efficiency were also investigated. The in vitro photocytotoxicity was evaluated using human colon adenocarcinoma cell line HT-29 and revealed time and concentration dependent cell death, consistent with a gradual release pattern of pTHPP over 24 h. This study is the first demonstration using bacterially derived P(HB-HV) copolymers for nanoparticle delivery of a hydrophobic photosensitizer drug and their potential application in PDT.Entities:
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Year: 2015 PMID： 26712706 DOI： 10.1007/s10856-015-5655-4
Source DB: PubMed Journal: J Mater Sci Mater Med ISSN： 0957-4530 Impact factor: 3.896