BACKGROUND/AIM: Plasma electrolytic oxidation (PEO), also known as micro-arc oxidation, is a promising electrochemical surface treatment technique for metals which has been used for the generation of various material surfaces and has been the focus of recent biomaterial research. It has been hypothesized that rough PEO surfaces should generally have properties that support cellular attachment and proliferation. However, this has not yet been demonstrated in systematically conducted studies. The present study investigated fibroblast cell proliferation and attachment to ground, electric discharge machining (EDM) and PEO-treated titanium surfaces differing in roughness and porosity. MATERIALS AND METHODS: Three surface variants with 'smoother', 'medium-coarse' and 'rough' surface topographies were generated by PEO and EDM on specimens of titanium alloy (with 6 wt% aluminum and 4 wt% vanadium) for comparison with more smoothly ground specimens. The in vitro effects on cellular attachment and proliferation were determined in 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), 5-bromo-2'-deoxyuridine (BrdU) and live/dead staining assays with L929 fibroblasts cultivated directly on the metal specimens. Cytocompatibility was determined in accordance with DIN 10993-5/-12 regulations by extract assays. RESULTS: Besides cytocompatibility, all PEO specimens exhibited similar biocompatibility and attachment properties, with vital, spindle-shaped adherent cells growing on the surface, regardless of their surface topology. There were no significant differences in cellular proliferation between the different surfaces and negative controls (cells growing in cell-culture plates). DISCUSSION/ CONCLUSION: With no differences in cellular proliferation and attachment between PEO surfaces with different roughness, we find no evidence to support the notion that rougher PEO surfaces are more favorable for cellular growth of fibroblasts in vitro.
BACKGROUND/AIM: Plasma electrolytic oxidation (PEO), also known as micro-arc oxidation, is a promising electrochemical surface treatment technique for metals which has been used for the generation of various material surfaces and has been the focus of recent biomaterial research. It has been hypothesized that rough PEO surfaces should generally have properties that support cellular attachment and proliferation. However, this has not yet been demonstrated in systematically conducted studies. The present study investigated fibroblast cell proliferation and attachment to ground, electric discharge machining (EDM) and PEO-treated titanium surfaces differing in roughness and porosity. MATERIALS AND METHODS: Three surface variants with 'smoother', 'medium-coarse' and 'rough' surface topographies were generated by PEO and EDM on specimens of titanium alloy (with 6 wt% aluminum and 4 wt% vanadium) for comparison with more smoothly ground specimens. The in vitro effects on cellular attachment and proliferation were determined in 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), 5-bromo-2'-deoxyuridine (BrdU) and live/dead staining assays with L929 fibroblasts cultivated directly on the metal specimens. Cytocompatibility was determined in accordance with DIN 10993-5/-12 regulations by extract assays. RESULTS: Besides cytocompatibility, all PEO specimens exhibited similar biocompatibility and attachment properties, with vital, spindle-shaped adherent cells growing on the surface, regardless of their surface topology. There were no significant differences in cellular proliferation between the different surfaces and negative controls (cells growing in cell-culture plates). DISCUSSION/ CONCLUSION: With no differences in cellular proliferation and attachment between PEO surfaces with different roughness, we find no evidence to support the notion that rougher PEO surfaces are more favorable for cellular growth of fibroblasts in vitro.
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Authors: Philip Hartjen; Alexia Hoffmann; Anders Henningsen; Mike Barbeck; Alexander Kopp; Lan Kluwe; Clarissa Precht; Olivia Quatela; Robert Gaudin; Max Heiland; Reinhard E Friedrich; Christian Knipfer; Daniel Grubeanu; Ralf Smeets; Ole Jung Journal: In Vivo Date: 2018 Mar-Apr Impact factor: 2.155
Authors: Mike Barbeck; Lennart Kühnel; Frank Witte; Jens Pissarek; Clarissa Precht; Xin Xiong; Rumen Krastev; Nils Wegner; Frank Walther; Ole Jung Journal: Int J Mol Sci Date: 2020-04-28 Impact factor: 5.923
Authors: Lyudmila V Parfenova; Elena S Lukina; Zulfia R Galimshina; Guzel U Gil'fanova; Veta R Mukaeva; Ruzil G Farrakhov; Ksenia V Danilko; Grigory S Dyakonov; Evgeny V Parfenov Journal: Molecules Date: 2020-01-06 Impact factor: 4.411
Authors: Ole Jung; Dario Porchetta; Marie-Luise Schroeder; Martin Klein; Nils Wegner; Frank Walther; Frank Feyerabend; Mike Barbeck; Alexander Kopp Journal: Int J Mol Sci Date: 2019-09-30 Impact factor: 5.923