Yong Yang1, Da-Peng Li2, Na Shen3, Xiao-Cheng Yu1, Jie-Bao Li1, Qi Song1, Jia-Heng Zhang4. 1. Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China. 2. Department of Head and Neck Surgery, Thyroid and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300000, China. 3. Department of Breast and Thyroid Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan, Hubei 430000, China. 4. Department of Breast and Thyroid Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China. Electronic address: zjh2396@163.com.
Abstract
OBJECTIVE: To investigate the expression of targeting protein for Xenopus kinesin-like protein 2 (TPX2) in breast cancer tissue and to explore its role in proliferation, migration and invasion of breast cancer cells. METHODS: The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot. The effect of TPX2 with RNA interference on proliferation, invasion and migration of breast cancer cells was observed by MTT and Transwell assays. RESULTS: Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue. TPX2 expression was also upregulated in breast cancer cell lines, and the TPX2 interfered by small interfering RNA could inhibit the proliferation, invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9. CONCLUSIONS: Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells, and contributes to promote the proliferation, migration and invasion of breast cancer cells.
OBJECTIVE: To investigate the expression of targeting protein for Xenopus kinesin-like protein 2 (TPX2) in breast cancer tissue and to explore its role in proliferation, migration and invasion of breast cancer cells. METHODS: The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot. The effect of TPX2 with RNA interference on proliferation, invasion and migration of breast cancer cells was observed by MTT and Transwell assays. RESULTS: Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue. TPX2 expression was also upregulated in breast cancer cell lines, and the TPX2 interfered by small interfering RNA could inhibit the proliferation, invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9. CONCLUSIONS: Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells, and contributes to promote the proliferation, migration and invasion of breast cancer cells.
Authors: Nishanth Ulhas Nair; Avinash Das; Vasiliki-Maria Rogkoti; Michiel Fokkelman; Richard Marcotte; Chiaro G de Jong; Esmee Koedoot; Joo Sang Lee; Isaac Meilijson; Sridhar Hannenhalli; Benjamin G Neel; Bob van de Water; Sylvia E Le Dévédec; Eytan Ruppin Journal: Sci Rep Date: 2019-07-29 Impact factor: 4.379