Literature DB >> 26699700

Comparison of Multilocus Sequence Typing and the Xpert C. difficile/Epi Assay for Identification of Clostridium difficile 027/NAP1/BI.

Tracy McMillen1, Mini Kamboj2, N Esther Babady3.   

Abstract

Clostridium difficile 027/NAP1/BI is the most common C. difficile strain in the United States. The Xpert C. difficile/Epi assay allows rapid, presumptive identification of C. difficile NAP1. We compared Xpert C. difficile/Epi to multilocus sequence typing for identification of C. difficile NAP1 and found "very good" agreement at 97.9% (κ = 0.86; 95% confidence interval, 0.80 to 0.91).
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID: 26699700      PMCID: PMC4767941          DOI: 10.1128/JCM.03075-15

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  18 in total

1.  An epidemic, toxin gene-variant strain of Clostridium difficile.

Authors:  L Clifford McDonald; George E Killgore; Angela Thompson; Robert C Owens; Sophia V Kazakova; Susan P Sambol; Stuart Johnson; Dale N Gerding
Journal:  N Engl J Med       Date:  2005-12-01       Impact factor: 91.245

2.  Rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples.

Authors:  Henrik Hasman; Dhany Saputra; Thomas Sicheritz-Ponten; Ole Lund; Christina Aaby Svendsen; Niels Frimodt-Møller; Frank M Aarestrup
Journal:  J Clin Microbiol       Date:  2013-10-30       Impact factor: 5.948

3.  Burden of Clostridium difficile infection in the United States.

Authors:  Fernanda C Lessa; Lisa G Winston; L Clifford McDonald
Journal:  N Engl J Med       Date:  2015-06-11       Impact factor: 91.245

4.  Multilocus sequence typing for Clostridium difficile.

Authors:  Ludovic Lemée; Jean-Louis Pons
Journal:  Methods Mol Biol       Date:  2010

5.  Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing.

Authors:  P Bidet; F Barbut; V Lalande; B Burghoffer; J C Petit
Journal:  FEMS Microbiol Lett       Date:  1999-06-15       Impact factor: 2.742

6.  Comparison of strain typing results for Clostridium difficile isolates from North America.

Authors:  Fred C Tenover; Thomas Akerlund; Dale N Gerding; Richard V Goering; Therése Boström; Anna-Maria Jonsson; Edith Wong; Alan T Wortman; David H Persing
Journal:  J Clin Microbiol       Date:  2011-03-09       Impact factor: 5.948

7.  Evaluation of the Cepheid Xpert Clostridium difficile Epi assay for diagnosis of Clostridium difficile infection and typing of the NAP1 strain at a cancer hospital.

Authors:  N Esther Babady; Jeffrey Stiles; Phyllis Ruggiero; Perminder Khosa; David Huang; Susan Shuptar; Mini Kamboj; Timothy E Kiehn
Journal:  J Clin Microbiol       Date:  2010-10-13       Impact factor: 5.948

8.  Update of Clostridium difficile infection due to PCR ribotype 027 in Europe, 2008.

Authors:  E J Kuijper; F Barbut; J S Brazier; N Kleinkauf; T Eckmanns; M L Lambert; D Drudy; F Fitzpatrick; C Wiuff; D J Brown; J E Coia; H Pituch; P Reichert; J Even; J Mossong; A F Widmer; K E Olsen; F Allerberger; D W Notermans; M Delmée; B Coignard; M Wilcox; B Patel; R Frei; E Nagy; E Bouza; M Marin; T Akerlund; A Virolainen-Julkunen; O Lyytikäinen; S Kotila; A Ingebretsen; B Smyth; P Rooney; I R Poxton; D L Monnet
Journal:  Euro Surveill       Date:  2008-07-31

9.  Multilocus sequence typing of Clostridium difficile.

Authors:  David Griffiths; Warren Fawley; Melina Kachrimanidou; Rory Bowden; Derrick W Crook; Rowena Fung; Tanya Golubchik; Rosalind M Harding; Katie J M Jeffery; Keith A Jolley; Richard Kirton; Tim E Peto; Gareth Rees; Nicole Stoesser; Alison Vaughan; A Sarah Walker; Bernadette C Young; Mark Wilcox; Kate E Dingle
Journal:  J Clin Microbiol       Date:  2009-12-30       Impact factor: 5.948

10.  C. difficile ribotype 027 or 176?

Authors:  Marcela Krutova; Jana Matejkova; Otakar Nyc
Journal:  Folia Microbiol (Praha)       Date:  2014-06-27       Impact factor: 2.099

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  4 in total

1.  Potential of real-time PCR threshold cycle (CT) to predict presence of free toxin and clinically relevant C. difficile infection (CDI) in patients with cancer.

Authors:  Mini Kamboj; Jennifer Brite; Tracy McMillen; Elizabeth Robilotti; Alejandro Herrera; Kent Sepkowitz; N Esther Babady
Journal:  J Infect       Date:  2017-12-08       Impact factor: 6.072

2.  Evaluation of a Combined Multilocus Sequence Typing and Whole-Genome Sequencing Two-Step Algorithm for Routine Typing of Clostridioides difficile.

Authors:  Mini Kamboj; Tracy McMillen; Mustafa Syed; Hoi Yan Chow; Krupa Jani; Anoshe Aslam; Jennifer Brite; Brian Fanelli; Nur A Hasan; Manoj Dadlani; Lars Westblade; Ahmet Zehir; Matthew Simon; N Esther Babady
Journal:  J Clin Microbiol       Date:  2021-01-21       Impact factor: 5.948

3.  First genotypic characterization of toxigenic Clostridioides difficile in Lithuanian hospitals reveals the prevalence of the hypervirulent ribotype 027/ST1.

Authors:  Simona Tratulyte; Jolanta Miciuleviciene; Nomeda Kuisiene
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-07-20       Impact factor: 3.267

4.  Accuracy of Xpert Clostridium difficile assay for the diagnosis of Clostridium difficile infection: A meta analysis.

Authors:  Yuanyuan Bai; Xiaorong Sun; Yan Jin; Yueling Wang; Juan Li
Journal:  PLoS One       Date:  2017-10-09       Impact factor: 3.240

  4 in total

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