PROBLEM: IgG is transformed from maternal serum into the offspring as passive immunization by FcRn expressed in placenta and/or infant intestine. This study aimed to investigate the quantitative correlation between IgG and FcRn during gestation and lactating periods in rat. METHOD OF STUDY: ELISA was performed to determine the variation of maternal and offspring IgG. Western blot and RT-qPCR were carried out to characterize FcRn expression in placenta and infant intestine. RESULTS: Maternal serum IgG appeared higher in first 2 weeks of lactation. The embryo IgG was in accordance with the FcRn expression in placenta. During the post-natal, the serum IgG concentration in feta was obviously lower than maternal IgG on day 0 before uptaking colostrums and rapidly increased on day 1. CONCLUSION: These findings would provide clues for the endogenous transportation and exogenous administration of IgG for a better IgG intervention in offspring.
PROBLEM: IgG is transformed from maternal serum into the offspring as passive immunization by FcRn expressed in placenta and/or infant intestine. This study aimed to investigate the quantitative correlation between IgG and FcRn during gestation and lactating periods in rat. METHOD OF STUDY: ELISA was performed to determine the variation of maternal and offspring IgG. Western blot and RT-qPCR were carried out to characterize FcRn expression in placenta and infant intestine. RESULTS: Maternal serum IgG appeared higher in first 2 weeks of lactation. The embryo IgG was in accordance with the FcRn expression in placenta. During the post-natal, the serum IgG concentration in feta was obviously lower than maternal IgG on day 0 before uptaking colostrums and rapidly increased on day 1. CONCLUSION: These findings would provide clues for the endogenous transportation and exogenous administration of IgG for a better IgG intervention in offspring.
Authors: Natalia A Lozano; Alejandro Lozano; Vanina Marini; Ricardo J Saranz; Richard S Blumberg; Kristi Baker; Maria F Agresta; Marina F Ponzio Journal: Am J Reprod Immunol Date: 2018-05-10 Impact factor: 3.886