Ting Shuang1, Min Wang2, Yingying Zhou1, Cong Shi1. 1. Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 110004, China. 2. Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 110004, China. Electronic address: wm21st@hotmail.com.
Abstract
OBJECTIVE: This study aims to measure the expression and subcellular location of NF-κB1 and c-Rel protein in serous epithelial ovarian cancer (EOC), and to test the correlation between NF-κB1 and c-Rel expression and clinicopathological parameters, chemoresistance, and prognosis of serous EOC. METHODS: A total of 63 specimens of serous EOC patients meeting the inclusion criteria with complete follow-up data were enrolled in our study. The specimens were divided into chemo-resistant group and sensitive group. The expression and subcellular location of NF-κB1 and c-Rel were assessed in paraffin sections using immunohistochemistry. The relationship between NF-κB1 and c-Rel protein expression and pathological characteristics of serous EOC, chemoresistance, prognosis and survival time was analyzed. RESULTS: The positive nuclear staining of NF-κB1 and c-Rel were significantly higher in the chemo-resistant serous EOC specimens than that in chemo-sensitive group. Lymph node metastasis and the nuclear expression of NF-κB1 and c-Rel were independent risk factors associated with chemotherapy resistance of serous EOC. Nucleus NF-κB1 and c-Rel expression along with FIGO stage were independent risk factors that strongly correlated with prognosis of serous EOC. Western blot result showed the protein level of NF-κB1 and c-Rel was significantly higher in chemoresistant group compared with in sensitive group. CONCLUSIONS: Over-expression of nuclear NF-κB1 and c-Rel are strong risk factors associated with chemoresistance and the prognosis of serous epithelial ovarian cancer.
OBJECTIVE: This study aims to measure the expression and subcellular location of NF-κB1 and c-Rel protein in serous epithelial ovarian cancer (EOC), and to test the correlation between NF-κB1 and c-Rel expression and clinicopathological parameters, chemoresistance, and prognosis of serous EOC. METHODS: A total of 63 specimens of serous EOC patients meeting the inclusion criteria with complete follow-up data were enrolled in our study. The specimens were divided into chemo-resistant group and sensitive group. The expression and subcellular location of NF-κB1 and c-Rel were assessed in paraffin sections using immunohistochemistry. The relationship between NF-κB1 and c-Rel protein expression and pathological characteristics of serous EOC, chemoresistance, prognosis and survival time was analyzed. RESULTS: The positive nuclear staining of NF-κB1 and c-Rel were significantly higher in the chemo-resistant serous EOC specimens than that in chemo-sensitive group. Lymph node metastasis and the nuclear expression of NF-κB1 and c-Rel were independent risk factors associated with chemotherapy resistance of serous EOC. Nucleus NF-κB1 and c-Rel expression along with FIGO stage were independent risk factors that strongly correlated with prognosis of serous EOC. Western blot result showed the protein level of NF-κB1 and c-Rel was significantly higher in chemoresistant group compared with in sensitive group. CONCLUSIONS: Over-expression of nuclear NF-κB1 and c-Rel are strong risk factors associated with chemoresistance and the prognosis of serous epithelial ovarian cancer.
Authors: Fiona E Yull; Alicia Beeghly-Fadiel; Demetra H Hufnagel; Andrew J Wilson; Jamie Saxon; Timothy S Blackwell; Jaclyn Watkins; Dineo Khabele; Marta A Crispens Journal: Biomark Res Date: 2020-09-15