Literature DB >> 26675461

Evaluating the Use of Commercial West Nile Virus Antigens as Positive Controls in the Rapid Analyte Measurement Platform West Nile Virus Assay.

Kristen L Burkhalter1, Harry M Savage1.   

Abstract

We evaluated the utility of 2 types of commercially available antigens as positive controls in the Rapid Analyte Measurement Platform (RAMP®) West Nile virus (WNV) assay. Purified recombinant WNV envelope antigens and whole killed virus antigens produced positive RAMP results and either type would be useful as a positive control. Killed virus antigens provide operational and economic advantages and we recommend their use over purified recombinant antigens. We also offer practical applications for RAMP positive controls and recommendations for preparing them.

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Keywords:  Rapid Analyte Measurement Platform® test; West Nile virus; commercial antigen; positive control

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Year:  2015        PMID： 26675461      PMCID： PMC7111257          DOI： 10.2987/moco-31-04-371-374.1

Source DB:  PubMed          Journal:  J Am Mosq Control Assoc        ISSN： 8756-971X            Impact factor:   0.917


  3 in total

Review 1.  Enzyme immunoassay techniques. An overview.

Authors:  T Porstmann; S T Kiessig
Journal:  J Immunol Methods       Date:  1992-06-24       Impact factor: 2.303

2.  Evaluation of commercial assays for detecting West Nile virus antigen.

Authors:  Kristen L Burkhalter; Robbin Lindsay; Robert Anderson; Antonia Dibernardo; Whalley Fong; Roger S Nasci
Journal:  J Am Mosq Control Assoc       Date:  2006-03       Impact factor: 0.917

3.  Evaluation of a rapid analyte measurement platform and real-time reverse-transcriptase polymerase chain reaction assay West Nile virus detection system in mosquito pools.

Authors:  Kristen L Burkhalter; Kalanthe Horiuchi; Brad J Biggerstaff; Harry M Savage; Roger S Nasci
Journal:  J Am Mosq Control Assoc       Date:  2014-03       Impact factor: 0.917
  3 in total

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