Kai-Feng Hung1, Chung-Ji Liu2, Peng-Chih Chiu3, Jiun-Sheng Lin2, Kuo-Wei Chang4, Wen-Yu Shih4, Shou-Yen Kao5, Hsi-Feng Tu6. 1. Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan. 2. Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan; Department of Dentistry, Taipei MacKay Memorial Hospital, Taipei, Taiwan. 3. Department of Dentistry, National Yang-Ming University Hospital, I-Lan, Taiwan. 4. Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. 5. Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. Electronic address: sykao@vghtpe.gov.tw. 6. Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan; Department of Dentistry, National Yang-Ming University Hospital, I-Lan, Taiwan. Electronic address: hftu@ym.edu.tw.
Abstract
OBJECTIVES: MicroRNAs (miRNAs, miRs) have shown diagnostic and prognostic potential for oral cancer but their role in oral potentially malignant disorder (OPMD) has been less investigated. We aimed to assess whether miR-21 and miR-31, two of the most relevant miRNAs in oral cancer, are useful as prognostic factors for OPMD progression. MATERIALS AND METHODS: miR-21 and miR-31 in 20 saliva samples and 46 tissue samples from patients with OPMD (mean follow-up of 820days) were analyzed by quantitative reverse transcription PCR and in situ hybridization, respectively. The log-rank test, receiver operating characteristic curve, and Kaplan-Meier disease free survival analysis were used to assess the correlation between miRNA levels and OPMD progression. RESULTS: Significantly increased salivary miR-21 and miR-31 expression (P=0.003 and P<0.001, respectively) was observed in patients with OPMD compared to control individuals. Patients with recurrent OPMD and/or malignant transformation exhibited a further augmented expression of miR-31, but not miR-21, in the epithelium. Furthermore, increased miR-31 expression as well as epithelial dysplasia is an independent risk factor for OPMD progression as demonstrated in Cox-proportional hazard model (HR: 8.43, P<0.05, 95%CI: 1.04 to 68.03). CONCLUSIONS: Salivary miR-21 and miR-31 are applicable as useful OPMD screening tools. Epithelial dysplasia and miR-31 up-regulation synergistically predict the increased incidence of recurrence and/or malignant transformation in patients with OPMD. Detection of miR-31 expression is an adjuvant method for screening of high-risk OPMD.
OBJECTIVES: MicroRNAs (miRNAs, miRs) have shown diagnostic and prognostic potential for oral cancer but their role in oral potentially malignant disorder (OPMD) has been less investigated. We aimed to assess whether miR-21 and miR-31, two of the most relevant miRNAs in oral cancer, are useful as prognostic factors for OPMD progression. MATERIALS AND METHODS:miR-21 and miR-31 in 20 saliva samples and 46 tissue samples from patients with OPMD (mean follow-up of 820days) were analyzed by quantitative reverse transcription PCR and in situ hybridization, respectively. The log-rank test, receiver operating characteristic curve, and Kaplan-Meier disease free survival analysis were used to assess the correlation between miRNA levels and OPMD progression. RESULTS: Significantly increased salivary miR-21 and miR-31 expression (P=0.003 and P<0.001, respectively) was observed in patients with OPMD compared to control individuals. Patients with recurrent OPMD and/or malignant transformation exhibited a further augmented expression of miR-31, but not miR-21, in the epithelium. Furthermore, increased miR-31 expression as well as epithelial dysplasia is an independent risk factor for OPMD progression as demonstrated in Cox-proportional hazard model (HR: 8.43, P<0.05, 95%CI: 1.04 to 68.03). CONCLUSIONS: Salivary miR-21 and miR-31 are applicable as useful OPMD screening tools. Epithelial dysplasia and miR-31 up-regulation synergistically predict the increased incidence of recurrence and/or malignant transformation in patients with OPMD. Detection of miR-31 expression is an adjuvant method for screening of high-risk OPMD.
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Mario Alovisi; Riccardo Aiuto; Daniele Garcovich; Vito Crincoli; Luigi Laino; Angela Pia Cazzolla; Giorgia Apollonia Caloro; Michele Di Cosola; Andrea Ballini; Lorenzo Lo Muzio; Giuseppe Troiano Journal: Int J Environ Res Public Health Date: 2022-04-27 Impact factor: 4.614
Authors: Maria Menini; Emanuele De Giovanni; Francesco Bagnasco; Francesca Delucchi; Francesco Pera; Domenico Baldi; Paolo Pesce Journal: J Pers Med Date: 2021-02-04