Shu-Chun Lin1,2,3, Chung-Ji Liu2,4, Si-Hua Ji1, Wan-Wen Hung1, Ying-Chieh Liu1, Sih-Rou Chang1, Hsi-Feng Tu1,2,5, Kuo-Wei Chang6,7,8. 1. Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan. 2. Department of Dentistry, College of Dentistry, National Yang Ming Chiao Tung University, Li-Nong St, No 155, Beitou, Taipei, Taiwan, 11211. 3. Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. 4. Department of Dentistry, Taipei MacKay Memorial Hospital, Taipei, Taiwan. 5. Department of Dentistry, National Yang Ming Chiao Tung Hospital, Yi-Lan, Taiwan. 6. Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan. ckcw@nycu.edu.tw. 7. Department of Dentistry, College of Dentistry, National Yang Ming Chiao Tung University, Li-Nong St, No 155, Beitou, Taipei, Taiwan, 11211. ckcw@nycu.edu.tw. 8. Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. ckcw@nycu.edu.tw.
Abstract
OBJECTIVES: Oncogenic miRNAs upregulated in OSCC play a range of versatile roles in oral carcinogenesis. Oral potentially malignant disorders (OPMDs) are the antecedent lesions to oral squamous carcinoma (OSCC) and they require a definitive diagnosis and early intervention. This study hypothesizes the presence of aberrant oncogenic miRNA expression in swabbed oral lesions. MATERIALS AND METHODS: The expression of miR-21, miR-31, miR-134, miR-146a, and miR-211 in swabbed samples from 36 dysplastic or hyperplastic OPMDs and 10 OSCCs, relative to respective normal mucosa within the same patient, is analyzed with qRT-PCR to develop a diagnosis. RESULTS: Upregulation of all tested miRNAs in OPMD and OSCC samples comparing to controls is found to have occurred. Receiver operating characteristics curve analysis shows that miR-31 gives the best diagnostic accuracy of 0.91 when differentiating OPMD/OSCC from controls. An analysis of miR-134 and miR-211 expression allows the discrimination of the dysplastic state associated with OPMD, while the use of expression of the combined miRNAs further improves the analytical performances when identifying the dysplastic state. The concordant upregulation of miR-21, miR-31, and miR-146a is found to occur during an early stage of OSCC carcinogenesis. CONCLUSION: This study demonstrates the upregulation of multiple oncogenic miRNAs in swabbed OPMD and OSCC samples. miRNA expression in swabbed collectives enables the differentiation between normal mucosa and OPMD/OSCC, independent of their histopathological severity. CLINICAL RELEVANCE: This conventional and convenient sampling tool, when coupled with an assessment of miR-31 expression, would seem to be an adjuvant approach to the diagnosis of OPMD and OSCC.
OBJECTIVES: Oncogenic miRNAs upregulated in OSCC play a range of versatile roles in oral carcinogenesis. Oral potentially malignant disorders (OPMDs) are the antecedent lesions to oral squamous carcinoma (OSCC) and they require a definitive diagnosis and early intervention. This study hypothesizes the presence of aberrant oncogenic miRNA expression in swabbed oral lesions. MATERIALS AND METHODS: The expression of miR-21, miR-31, miR-134, miR-146a, and miR-211 in swabbed samples from 36 dysplastic or hyperplastic OPMDs and 10 OSCCs, relative to respective normal mucosa within the same patient, is analyzed with qRT-PCR to develop a diagnosis. RESULTS: Upregulation of all tested miRNAs in OPMD and OSCC samples comparing to controls is found to have occurred. Receiver operating characteristics curve analysis shows that miR-31 gives the best diagnostic accuracy of 0.91 when differentiating OPMD/OSCC from controls. An analysis of miR-134 and miR-211 expression allows the discrimination of the dysplastic state associated with OPMD, while the use of expression of the combined miRNAs further improves the analytical performances when identifying the dysplastic state. The concordant upregulation of miR-21, miR-31, and miR-146a is found to occur during an early stage of OSCC carcinogenesis. CONCLUSION: This study demonstrates the upregulation of multiple oncogenic miRNAs in swabbed OPMD and OSCC samples. miRNA expression in swabbed collectives enables the differentiation between normal mucosa and OPMD/OSCC, independent of their histopathological severity. CLINICAL RELEVANCE: This conventional and convenient sampling tool, when coupled with an assessment of miR-31 expression, would seem to be an adjuvant approach to the diagnosis of OPMD and OSCC.
Authors: Angela B Y Hui; Michelle Lenarduzzi; Tiffaney Krushel; Levi Waldron; Melania Pintilie; Wei Shi; Bayardo Perez-Ordonez; Igor Jurisica; Brian O'Sullivan; John Waldron; Pat Gullane; Bernard Cummings; Fei-Fei Liu Journal: Clin Cancer Res Date: 2010-02-09 Impact factor: 12.531
Authors: Scott Langevin; Damaris Kuhnell; Tess Parry; Jacek Biesiada; Shouxiong Huang; Trisha Wise-Draper; Keith Casper; Xiang Zhang; Mario Medvedovic; Susan Kasper Journal: Oncotarget Date: 2017-07-27