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Literature DB >> 266715

Dual role of interfacial phospholipid in phospholipase A2 catalysis.

Abstract

The results of crosslinking experiments with dimethyl suberimidate and gel filtration binding studies are used to delineate a detailed model for phospholipase A2(phosphatide 2-acyl-hydrolase, EC 3.1.1.4) action in the presence of Ca2+ on mixed micelles of Triton X-100 and phospholipid. Important features of the "dual-phospholipid" model are: (i) the use of the nonionic surfactant as an inert matrix that may influence lipid conformation but does not interact with the enzyme; (ii) the involvement of two lipid molecules in a single cycle of catalysis as an explanation for the "surface dilution" phenomenon; (iii) the requirement of an ordered reaction whereby divalent metal ion binds prior to phospholipid binding; and (iv) the induction by lipid substrate of an asymmetric dimer structure for the enzyme.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1977 PMID： 266715 PMCID： PMC431050 DOI： 10.1073/pnas.74.5.1950

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. Analysis of phospholipase C (Bacillus cereus) action toward mixed micelles of phospholipid and surfactant.

Authors: B R Eaton; E A Dennis
Journal: Arch Biochem Biophys Date: 1976-10 Impact factor: 4.013

2. Measurement of protein-binding phenomena by gel filtration.

Authors: J P HUMMEL; W J DREYER
Journal: Biochim Biophys Acta Date: 1962-10-08

3. The interaction of phospholipase A2 with micellar interfaces. The role of the N-terminal region.

Authors: M C van Dam-Mieras; A J Slotboom; W A Pieterson; G H de Haas
Journal: Biochemistry Date: 1975-12-16 Impact factor: 3.162

4. Phospholipase A2 activity towards phosphatidylcholine in mixed micelles: surface dilution kinetics and the effect of thermotropic phase transitions.

Authors: E A Dennis
Journal: Arch Biochem Biophys Date: 1973-10 Impact factor: 4.013

5. The mechanism of interfacial activation of phospholipase A2.

Authors: M A Wells
Journal: Biochemistry Date: 1974-05-21 Impact factor: 3.162

6. Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.

Authors: G E Davies; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

7. Kinetic dependence of phospholipase A 2 activity on the detergent Triton X-100.

Authors: E A Dennis
Journal: J Lipid Res Date: 1973-03 Impact factor: 5.922

8. Studies on phospholipase A and its zymogen from porcine pancreas. IV. The influence of chemical modification of the lecithin structure on substrate properties.

Authors: P P Bonsen; G H de Haas; W A Pieterson; L L van Deenen
Journal: Biochim Biophys Acta Date: 1972-07-07

9. Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations.

Authors: K Jacobson; D Papahadjopoulos
Journal: Biochemistry Date: 1975-01-14 Impact factor: 3.162

10. Chemical modification of the histidine residue in phospholipase A2 (Naja naja naja). A case of half-site reactivity.

Authors: M F Roberts; R A Deems; T C Mincey; E A Dennis
Journal: J Biol Chem Date: 1977-04-10 Impact factor: 5.157

19 in total

Review 1. Phospholipase A₂ catalysis and lipid mediator lipidomics.

Authors: Varnavas D Mouchlis; Edward A Dennis
Journal: Biochim Biophys Acta Mol Cell Biol Lipids Date: 2018-08-23 Impact factor: 4.698

2. Sequence-induced trimerization of phospholipase A2: structure of a trimeric isoform of PLA2 from common krait (Bungarus caeruleus) at 2.5 A resolution.

Authors: Garima Singh; S Gourinath; K Saravanan; Sujata Sharma; S Bhanumathi; Ch Betzel; A Srinivasan; T P Singh
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2004-10-16

3. The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei.

Authors: Gregory S Richmond; Terry K Smith
Journal: Biochem J Date: 2007-07-15 Impact factor: 3.857

4. Oxygenation of 1-docosahexaenoyl lysophosphatidylcholine by lipoxygenases; conjugated hydroperoxydiene and dihydroxytriene derivatives.

Authors: Long Shuang Huang; Mee Ree Kim; Dai-Eun Sok
Journal: Lipids Date: 2007-09-19 Impact factor: 1.880

5. Cobra venom phospholipase A2: a review of its action toward lipid/water interfaces.

Authors: E A Dennis; P L Darke; R A Deems; C R Kensil; A Plückthun
Journal: Mol Cell Biochem Date: 1981-04-13 Impact factor: 3.396

6. An examination of structural interactions presumed to be of importance in the stabilization of phospholipase A2 dimers based upon comparative protein sequence analysis of a monomeric and dimeric enzyme from the venom of Agkistrodon p. piscivorus.

Authors: W Welches; I Reardon; R L Heinrikson
Journal: J Protein Chem Date: 1993-04

7. Phospholipase A2 contamination of cobra venom factor preparations. Biologic role in complement-dependent in vivo reactions and inactivation with p-bromophenacyl bromide.

Authors: J O Shaw; M F Roberts; R J Ulevitch; P Henson; E A Dennis
Journal: Am J Pathol Date: 1978-06 Impact factor: 4.307

Dual role of interfacial phospholipid in phospholipase A2 catalysis.

1. Analysis of phospholipase C (Bacillus cereus) action toward mixed micelles of phospholipid and surfactant.

2. Measurement of protein-binding phenomena by gel filtration.

3. The interaction of phospholipase A2 with micellar interfaces. The role of the N-terminal region.

4. Phospholipase A2 activity towards phosphatidylcholine in mixed micelles: surface dilution kinetics and the effect of thermotropic phase transitions.

5. The mechanism of interfacial activation of phospholipase A2.

6. Use of dimethyl suberimidate, a cross-linking reagent, in studying the subunit structure of oligomeric proteins.

7. Kinetic dependence of phospholipase A 2 activity on the detergent Triton X-100.

8. Studies on phospholipase A and its zymogen from porcine pancreas. IV. The influence of chemical modification of the lecithin structure on substrate properties.

9. Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations.

10. Chemical modification of the histidine residue in phospholipase A2 (Naja naja naja). A case of half-site reactivity.

Review 1. Phospholipase A₂ catalysis and lipid mediator lipidomics.

2. Sequence-induced trimerization of phospholipase A2: structure of a trimeric isoform of PLA2 from common krait (Bungarus caeruleus) at 2.5 A resolution.

3. The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei.

4. Oxygenation of 1-docosahexaenoyl lysophosphatidylcholine by lipoxygenases; conjugated hydroperoxydiene and dihydroxytriene derivatives.

5. Cobra venom phospholipase A2: a review of its action toward lipid/water interfaces.

6. An examination of structural interactions presumed to be of importance in the stabilization of phospholipase A2 dimers based upon comparative protein sequence analysis of a monomeric and dimeric enzyme from the venom of Agkistrodon p. piscivorus.

7. Phospholipase A2 contamination of cobra venom factor preparations. Biologic role in complement-dependent in vivo reactions and inactivation with p-bromophenacyl bromide.

8. Structural importance of the acyl group in substrate specificity of purified bovine lysophospholipase D.

9. Lipidomics-based assays coupled with computational approaches can identify novel phospholipase A₂ inhibitors.

10. Inactivation of secretory phospholipase A2 by ionizing radiation.

Review 1. Phospholipase A2 catalysis and lipid mediator lipidomics.

Review 1. Phospholipase A₂ catalysis and lipid mediator lipidomics.