Yibin Zhuang1,2, Jingjie Jiang1,3, Huiping Bi1,2, Hua Yin1,2, Shaowei Liu4, Tao Liu5,6. 1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. 2. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. 3. Department of Food Science and Technology, School of Biological Engineering, East China University of Science and Technology, Shanghai, 200237, China. 4. Department of Food Science and Technology, School of Biological Engineering, East China University of Science and Technology, Shanghai, 200237, China. swliu@ecust.edu.cn. 5. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. liu_t@tib.cas.cn. 6. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. liu_t@tib.cas.cn.
Abstract
OBJECTIVES: To produce rosmarinic acid analogues in the recombinant Escherichia coli BLRA1, harboring a 4-coumarate: CoA ligase from Arabidopsis thaliana (At4CL) and a rosmarinic acid synthase from Coleus blumei (CbRAS). RESULTS: Incubation of the recombinant E. coli strain BLRA1 with exogenously supplied phenyllactic acid (PL) and analogues as acceptor substrates, and coumaric acid and analogues as donor substrates led to production of 18 compounds, including 13 unnatural RA analogues. CONCLUSION: This work demonstrates the viability of synthesizing a broad range of rosmarinic acid analogues in E. coli, and sheds new light on the substrate specificity of CbRAS.
OBJECTIVES: To produce rosmarinic acid analogues in the recombinant Escherichia coli BLRA1, harboring a 4-coumarate: CoA ligase from Arabidopsis thaliana (At4CL) and a rosmarinic acid synthase from Coleus blumei (CbRAS). RESULTS: Incubation of the recombinant E. coli strain BLRA1 with exogenously supplied phenyllactic acid (PL) and analogues as acceptor substrates, and coumaric acid and analogues as donor substrates led to production of 18 compounds, including 13 unnatural RA analogues. CONCLUSION: This work demonstrates the viability of synthesizing a broad range of rosmarinic acid analogues in E. coli, and sheds new light on the substrate specificity of CbRAS.
Authors: Aymerick Eudes; Maxence Mouille; David S Robinson; Veronica T Benites; George Wang; Lucien Roux; Yi-Lin Tsai; Edward E K Baidoo; Tsan-Yu Chiu; Joshua L Heazlewood; Henrik V Scheller; Aindrila Mukhopadhyay; Jay D Keasling; Samuel Deutsch; Dominique Loqué Journal: Microb Cell Fact Date: 2016-11-21 Impact factor: 5.328