| Literature DB >> 26660176 |
Shuang Deng1,2, Michael Kiscoan1, Clint Frazee1, Susan Abdel-Rahman2, Jignesh Dalal2, Uttam Garg3.
Abstract
Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of <10 %.Entities:
Keywords: Bone marrow transplant; Busulfan; Leukemia; Liquid chromatography; Mass spectrometry
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Year: 2016 PMID： 26660176 DOI： 10.1007/978-1-4939-3252-8_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745