Literature DB >> 26660081

Application of Differential Proteomic Analysis to Authenticate Ophiocordyceps sinensis.

Shiwei Zhang1, Xintian Lai2, Bifang Li1, Cong Wu1, Shifeng Wang1, Xuejian Chen1, Jingmin Huang1, Guowu Yang1.   

Abstract

Ophiocordyceps sinensis (Berk.) Sacc. is one of the most well-known fungi in traditional Chinese medicine and is attracting attention because of its nutritious and medicinal properties. The present study aimed to produce a proteomic map to identify common O. sinensis proteins. The caterpillar body and stroma of O. sinensis collected from five locations and four fungal specimens of similar appearance were examined by two-dimensional electrophoresis (2-DE). Five proteins were identified using MALDI-TOF--TOF/MS, and the 2-DE identification pattern was provided. OCS_04585 and β-lactamase domain-containing protein, the two abundant and characteristic proteins, were separated and purified using liquid-phase isoelectric focusing. The products were high-quality materials that can be used for future protein-function studies and immunoassay development.

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Year:  2015        PMID： 26660081     DOI： 10.1007/s00284-015-0950-3

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  20 in total

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Authors:  H Katayama; T Nagasu; Y Oda
Journal:  Rapid Commun Mass Spectrom       Date:  2001       Impact factor: 2.419

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Journal:  J Agric Food Chem       Date:  2006-04-19       Impact factor: 5.279

4.  Global analysis of a plasmid-cured Shigella flexneri strain: new insights into the interaction between the chromosome and a virulence plasmid.

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5.  Methods for mucin analysis: a comparative study.

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Journal:  J Agric Food Chem       Date:  2009-07-22       Impact factor: 5.279

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Authors:  Xuanwei Zhou; Zhenghua Gong; Ying Su; Juan Lin; Kexuan Tang
Journal:  J Pharm Pharmacol       Date:  2009-03       Impact factor: 3.765

7.  Phylogenetic classification of Cordyceps and the clavicipitaceous fungi.

Authors:  Gi-Ho Sung; Nigel L Hywel-Jones; Jae-Mo Sung; J Jennifer Luangsa-Ard; Bhushan Shrestha; Joseph W Spatafora
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8.  Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.

Authors:  C H O'Callaghan; A Morris; S M Kirby; A H Shingler
Journal:  Antimicrob Agents Chemother       Date:  1972-04       Impact factor: 5.191

9.  DNA barcoding the commercial Chinese caterpillar fungus.

Authors:  Li Xiang; Jingyuan Song; Tianyi Xin; Yingjie Zhu; Linchun Shi; Xiaolan Xu; Xiaohui Pang; Hui Yao; Wenjia Li; Shilin Chen
Journal:  FEMS Microbiol Lett       Date:  2013-08-28       Impact factor: 2.742

10.  Altered proteomic polymorphisms in the caterpillar body and stroma of natural Cordyceps sinensis during maturation.

Authors:  Yun-Zi Dong; Li-Juan Zhang; Zi-Mei Wu; Ling Gao; Yi-Sang Yao; Ning-Zhi Tan; Jian-Yong Wu; Luqun Ni; Jia-Shi Zhu
Journal:  PLoS One       Date:  2014-10-13       Impact factor: 3.240
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  5 in total

1.  Proteomic identification of marker proteins and its application to authenticate Ophiocordyceps sinensis.

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Journal:  3 Biotech       Date:  2018-05-08       Impact factor: 2.406

2.  Evaluation of the Use of TRIzol-Based Protein Extraction Approach for Gel-Based Proteomic Analysis of Dried Seafood Products and Chinese Tonic Foods.

Authors:  Kin-Ka Chan; Celia Sze-Nga Kwok; Eric Tung-Po Sze; Fred Wang-Fat Lee
Journal:  Int J Mol Sci       Date:  2018-07-09       Impact factor: 5.923

3.  Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics.

Authors:  Ping Zhang; Saina Li; Juan Li; Feng Wei; Xianlong Cheng; Guifeng Zhang; Shuangcheng Ma; Bin Liu
Journal:  Molecules       Date:  2018-04-26       Impact factor: 4.411

4.  Proteome sequencing and analysis of Ophiocordyceps sinensis at different culture periods.

Authors:  Bo Zhang; Bo Li; Xiao-Hui Men; Zhe-Wen Xu; Hui Wu; Xiang-Tian Qin; Feng Xu; Yi Teng; Shui-Jin Yuan; Li-Qun Jin; Zhi-Qiang Liu; Yu-Guo Zheng
Journal:  BMC Genomics       Date:  2020-12-11       Impact factor: 3.969

Review 5.  Chemometric-Guided Approaches for Profiling and Authenticating Botanical Materials.

Authors:  Evelyn J Abraham; Joshua J Kellogg
Journal:  Front Nutr       Date:  2021-11-26
  5 in total

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