Nurgul Ozcan1, Fatma Ucar2, Abdullah Ercan Arzuhal2, Erdem Bulut2, Alpaslan Ozturk2, Mine Taslipinar Yavuz3, Ismail Temel2, Gonul Erden4. 1. Diskapi Yildirim Beyazit Training and Research Hospital, Department of Clinical Biochemistry, Ankara, Turkey. Electronic address: nersoyozcan@gmail.com. 2. Diskapi Yildirim Beyazit Training and Research Hospital, Department of Clinical Biochemistry, Ankara, Turkey. 3. Hacettepe University, Faculty of Medicine, Department of Clinical Biochemistry, Ankara, Turkey. 4. Diskapi Yildirim Beyazit Training and Research Hospital, Department of Clinical Biochemistry, Ankara, Turkey; Hacettepe University, Faculty of Medicine, Department of Clinical Biochemistry, Ankara, Turkey.
Abstract
OBJECTIVES: We assessed the analytical performance of newly developed Access 25(OH) Vitamin D Total assay with Beckman Coulter Unicel DXI 800 and evaluated the agreement between a reference method liquid chromatography/tandem mass spectrometry (LC-MS/MS) and a chemiluminescence method (LIAISON, DiaSorin). DESIGN AND METHODS: 160 serum samples were included. Deming Regression analysis and Bland-Altman plots were used. The concordance correlation coefficient (CCC) was used to assess the degree of agreement between assays and the reference method. RESULTS: The CV% values of Unicel DXI 800 for within-run, between-run and between-day were lower than 6%. When compared to LC-MS/MS, the Access 25(OH) Vitamin D Total assay demonstrated an R value of 0.9444 (intercept -0.089, slope 0.951), with an average bias of -2.9%, and the LIAISON 25(OH) Vitamin D Total assay an R value of 0.9405 (intercept -0605, slope 0.924), with an average bias of -13.6%. In comparison with the LIAISON 25(OH) Vitamin D Total assay, the Access 25(OH) Vitamin D Total assay demonstrated an R value of 0.9498 (intercept 0.528, slope 1.029), with an average bias of 1.2%. The agreement with the LC-MS/MS method, based on values of the CCC, was moderate for the Unicel DXI 800 and LIAISON method (0.95, 0.94 respectively). CONCLUSIONS: The new, automated Access 25(OH) Vitamin D Total assay showed an acceptable correlation with LC-MS/MS and LIAISON. Both methods moderately achieved to classify the patients according to their vitamin D status. However, we need further standardization of vitamin D assays to enhance the accuracy and comparability.
OBJECTIVES: We assessed the analytical performance of newly developed Access 25(OH) Vitamin D Total assay with Beckman Coulter UnicelDXI 800 and evaluated the agreement between a reference method liquid chromatography/tandem mass spectrometry (LC-MS/MS) and a chemiluminescence method (LIAISON, DiaSorin). DESIGN AND METHODS: 160 serum samples were included. Deming Regression analysis and Bland-Altman plots were used. The concordance correlation coefficient (CCC) was used to assess the degree of agreement between assays and the reference method. RESULTS: The CV% values of UnicelDXI 800 for within-run, between-run and between-day were lower than 6%. When compared to LC-MS/MS, the Access 25(OH) Vitamin D Total assay demonstrated an R value of 0.9444 (intercept -0.089, slope 0.951), with an average bias of -2.9%, and the LIAISON 25(OH) Vitamin D Total assay an R value of 0.9405 (intercept -0605, slope 0.924), with an average bias of -13.6%. In comparison with the LIAISON 25(OH) Vitamin D Total assay, the Access 25(OH) Vitamin D Total assay demonstrated an R value of 0.9498 (intercept 0.528, slope 1.029), with an average bias of 1.2%. The agreement with the LC-MS/MS method, based on values of the CCC, was moderate for the UnicelDXI 800 and LIAISON method (0.95, 0.94 respectively). CONCLUSIONS: The new, automated Access 25(OH) Vitamin D Total assay showed an acceptable correlation with LC-MS/MS and LIAISON. Both methods moderately achieved to classify the patients according to their vitamin D status. However, we need further standardization of vitamin D assays to enhance the accuracy and comparability.
Authors: Hanna Karla Andrade Guapyassú Machado; Carolina Steller Wagner Martins; Vanda Jorgetti; Rosilene Motta Elias; Rosa Maria Affonso Moysés Journal: J Bras Nefrol Date: 2019-09-26
Authors: Jindra Windrichova; Pavel Broz; Radka Fuchsova; Ondrej Topolcan; Ladislav Pecen; Otto Mayer; Radek Kucera Journal: J Med Biochem Date: 2021-06-05 Impact factor: 3.402