| Literature DB >> 26652454 |
Mahmoud Amouzadeh Tabrizi1, Mojtaba Shamsipur2, Ali Mostafaie3.
Abstract
Herein, an electrochemical human immunoglobulin G (HIgG) immunosensor was fabricated using overoxidized polypyrrole decorated with gold nanoparticle (OPPy-Aunano) modified screen printed electrode (OPPy-Aunano/SPE). The results clearly demonstrate that OPPy-Aunano film is very permeable to [Fe(CN)6](3-/4-). Therefore, the fabrication of immunosensor based on permeability of OPPy-Aunano film to [Fe(CN)6](3-/4-) probe is a novel strategy for the fabrication of label free electrochemical immunosensor. The electrochemical impedance spectroscopy (EIS) techniques are used for the determination of HIgG in the concentration range 0.5 to 125.0ngmL(-1) and with a limit of detection of 0.02ngmL(-1), respectively.Entities:
Keywords: EIS; HIgG; Immunosensor; Label-free method; OPPy-Au(nano)
Mesh:
Substances:
Year: 2015 PMID: 26652454 DOI: 10.1016/j.msec.2015.10.093
Source DB: PubMed Journal: Mater Sci Eng C Mater Biol Appl ISSN: 0928-4931 Impact factor: 7.328