Literature DB >> 26634335

Installation of a Rigid EDTA-Like Motif into a Protein α-Helix for Paramagnetic NMR Spectroscopy with Cobalt(II) Ions.

James D Swarbrick1, Phuc Ung2, Matthew L Dennis2, Michael D Lee2, Sandeep Chhabra2, Bim Graham3.   

Abstract

Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i+4 arrangement within a protein α-helix leads to generation of an EDTA-like metal ion-binding motif. Rigid binding of a Co(II) ion by this motif produces pseudo-contact shifts suitable for paramagnetic NMR structural studies.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  alpha-helix; cobalt; paramagnetic NMR spectroscopy; protein structures; pseudo-contact shifts

Mesh：

Substances：

Year:  2015        PMID： 26634335     DOI： 10.1002/chem.201503139

Source DB:  PubMed          Journal:  Chemistry        ISSN： 0947-6539            Impact factor:   5.236


  3 in total

1.  Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR.

Authors:  Leah M Seebald; Christopher M DeMott; Srivathsan Ranganathan; Papa Nii Asare-Okai; Anastasia Glazunova; Alan Chen; Alexander Shekhtman; Maksim Royzen
Journal:  J Inorg Biochem       Date:  2017-02-24       Impact factor: 4.155

Review 2.  Paramagnetic Chemical Probes for Studying Biological Macromolecules.

Authors:  Qing Miao; Christoph Nitsche; Henry Orton; Mark Overhand; Gottfried Otting; Marcellus Ubbink
Journal:  Chem Rev       Date:  2022-01-27       Impact factor: 72.087

Review 3.  A Double-Armed, Hydrophilic Transition Metal Complex as a Paramagnetic NMR Probe.

Authors:  Qing Miao; Wei-Min Liu; Thomas Kock; Anneloes Blok; Monika Timmer; Mark Overhand; Marcellus Ubbink
Journal:  Angew Chem Int Ed Engl       Date:  2019-08-13       Impact factor: 15.336
  3 in total

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