Hu Hao1, Sitao Li2, Wei Zhou3, Hong Wang4, Mengxian Liu2, Congcong Shi5, Jing Chen5, Xin Xiao1. 1. Department of Neonatology, Sixth Affiliated Hospital of Sun Yat-Sen University Guangzhou 510655, China ; Laboratory of Inborn Errors of Metabolism, Sixth Affiliated Hospital of Sun Yat-Sen University Guangzhou 510655, China. 2. Department of Neonatology, Sixth Affiliated Hospital of Sun Yat-Sen University Guangzhou 510655, China. 3. Department of Neonatology, Guangzhou Women and Children Medical Center Guangzhou 510623, China. 4. Department of Neonatology, Tianhe Women and Children Hospital Guangzhou 510620, China. 5. Laboratory of Inborn Errors of Metabolism, Sixth Affiliated Hospital of Sun Yat-Sen University Guangzhou 510655, China.
Abstract
OBJECTIVE: To characterize the metabolic products of urine associated with preterm birth, thus providing clinical guidelines for intestinal and parenteral nutrition in preterm infants. METHODS: Urine samples of 47 preterm infants and 45 full-term infants were collected and prepared for trimethylsilylation by treatment with urease. The levels of lysine, phenylalanine, histidine, ornithine, fumaric acid, malic acid, succinic acid, lactose, stearic acid, and 4-hydroxyphenylacetic acid were detected by gas chromatography-mass spectrometry (GC/MS), and statistically analyzed. RESULTS: The normalized concentrations of the following metabolites in preterm infant urine samples were significantly lower than that of full-term infant urine samples: lysine (P = 0.003), phenylalanine (P = 0.001), histidine (P = 0.006), ornithine (P = 0.000), fumaric acid (P = 0.002), malic acid (P = 0.006), succinic acid (P = 0.000), lactose (P = 0.000), stearic acid (P = 0.000) and 4-hydroxyphenylacetic acid (P = 0.000). CONCLUSIONS: The results of the GC/MS analysis indicated that amino acid, carbohydrate, and fatty acid metabolism defects exist in preterm infants. The use of GC/MS to determine metabolic products in urine samples could be helpful for prospectively evaluating the nutritional status of preterm infants, and therefore providing clinical guidelines on reasonable nutritional support.
OBJECTIVE: To characterize the metabolic products of urine associated with preterm birth, thus providing clinical guidelines for intestinal and parenteral nutrition in preterm infants. METHODS: Urine samples of 47 preterm infants and 45 full-term infants were collected and prepared for trimethylsilylation by treatment with urease. The levels of lysine, phenylalanine, histidine, ornithine, fumaric acid, malic acid, succinic acid, lactose, stearic acid, and 4-hydroxyphenylacetic acid were detected by gas chromatography-mass spectrometry (GC/MS), and statistically analyzed. RESULTS: The normalized concentrations of the following metabolites in preterm infant urine samples were significantly lower than that of full-term infant urine samples: lysine (P = 0.003), phenylalanine (P = 0.001), histidine (P = 0.006), ornithine (P = 0.000), fumaric acid (P = 0.002), malic acid (P = 0.006), succinic acid (P = 0.000), lactose (P = 0.000), stearic acid (P = 0.000) and 4-hydroxyphenylacetic acid (P = 0.000). CONCLUSIONS: The results of the GC/MS analysis indicated that amino acid, carbohydrate, and fatty acid metabolism defects exist in preterm infants. The use of GC/MS to determine metabolic products in urine samples could be helpful for prospectively evaluating the nutritional status of preterm infants, and therefore providing clinical guidelines on reasonable nutritional support.
Authors: Yasser F Ali; Salah El-Morshedy; Riad M Elsayed; Amr M El-Sherbini; Saber Am El-Sayed; Nasser Ismail A Abdelrahman; Abdulbasit Abdulhalim Imam Journal: Neuropsychiatr Dis Treat Date: 2017-04-19 Impact factor: 2.570
Authors: Luise V Marino; Simone Paulson; James J Ashton; Charlotte Weeks; Aneurin Young; John V Pappachan; Jonathan Swann; Mark J Johnson; Robert Mark Beattie Journal: Nutrients Date: 2022-09-23 Impact factor: 6.706