Dong Yuan1, Changqing Yu1, Chunyu Zeng1. 1. Department of Cardiology, Daping Hospital, The Third Military Medical University Chongqing 400042, China ; Chongqing Institute of Cardiology Chongqing 400042, China.
Abstract
OBJECTIVE: The study aimed to investigate the association between polymorphism of I-FABP gene and coronary heart disease (CHD). METHODS: 225 patients with CHD were randomly recruited into the case group, and 196 healthy elderly volunteers were recruited from Medical Examination Center of our hospital as control. General clinical data were collected and plasma TC, TG, LDL-C, HDL-C levels were measured. Besides, polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) technology were used to detect the polymorphism of Hha-I enzyme cleavage sites in I-FABP gene in the study population. RESULTS: Hha-I cleavage sites occurred at codon 54 in exon in the coding sequencing of I-FABP gene in all participants. After cleavage with Hha-I enzyme, the genotypes were identified as wild-type A/A, heterozygous mutant A/T and homozygous mutant T/T. In case group, A/T and T/T genetic carriers had significantly higher levels of TC, TG and LDL-C than A/A carriers (P<0.05). However, in control group, similar differences were not observed (P>0.05). BMI, dietary habits and I-FABP alleles were independent risk factors of CHD. CONCLUSION: The polymorphism of I-FABP gene existed in the study population. And this genetic variation had influence on lipid metabolism, which was associated with the risk of developing CHD. I-FABP gene polymorphism may contribute to the increased genetic susceptibility to CHD.
OBJECTIVE: The study aimed to investigate the association between polymorphism of I-FABP gene and coronary heart disease (CHD). METHODS: 225 patients with CHD were randomly recruited into the case group, and 196 healthy elderly volunteers were recruited from Medical Examination Center of our hospital as control. General clinical data were collected and plasma TC, TG, LDL-C, HDL-C levels were measured. Besides, polymerase chain reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) technology were used to detect the polymorphism of Hha-I enzyme cleavage sites in I-FABP gene in the study population. RESULTS: Hha-I cleavage sites occurred at codon 54 in exon in the coding sequencing of I-FABP gene in all participants. After cleavage with Hha-I enzyme, the genotypes were identified as wild-type A/A, heterozygous mutant A/T and homozygous mutant T/T. In case group, A/T and T/T genetic carriers had significantly higher levels of TC, TG and LDL-C than A/A carriers (P<0.05). However, in control group, similar differences were not observed (P>0.05). BMI, dietary habits and I-FABP alleles were independent risk factors of CHD. CONCLUSION: The polymorphism of I-FABP gene existed in the study population. And this genetic variation had influence on lipid metabolism, which was associated with the risk of developing CHD. I-FABP gene polymorphism may contribute to the increased genetic susceptibility to CHD.
Authors: Dominik Dytfeld; Joanna Leśna; Agnieszka Protasewicz; Wojciech Sarnowski; Wojciech Dyszkiewicz; Stanisław Paradowski Journal: Pol Arch Med Wewn Date: 2002-07
Authors: Rachel G Khadaroo; Spyridon Fortis; Saad Y Salim; Catherine Streutker; Thomas A Churchill; Haibo Zhang Journal: PLoS One Date: 2014-12-26 Impact factor: 3.240