Andrew J Percy1, Yassene Mohammed1,2, Juncong Yang1, Christoph H Borchers1,3. 1. University of Victoria - Genome British Columbia Proteomics Centre, Vancouver Island Technology Park, #3101-4464 Markham St., Victoria, BC V8Z 7X8, Canada. 2. Center for Proteomics & Metabolomics, Leiden University Medical Center, 2333 ZA, Leiden, The Netherlands. 3. Department of Biochemistry & Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada.
Abstract
BACKGROUND: An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma. DISCUSSION/ CONCLUSION: The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.
BACKGROUND: An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma. DISCUSSION/ CONCLUSION: The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.
Authors: Yassene Mohammed; Bart J van Vlijmen; Juncong Yang; Andrew J Percy; Magnus Palmblad; Christoph H Borchers; Frits R Rosendaal Journal: Blood Adv Date: 2017-06-20
Authors: Irina M Larina; Andrew J Percy; Juncong Yang; Christoph H Borchers; Andrei M Nosovsky; Anatoli I Grigoriev; Evgeny N Nikolaev Journal: Sci Rep Date: 2017-08-15 Impact factor: 4.379
Authors: Yassene Mohammed; Ruud S Kootte; Wil F Kopatz; Christoph H Borchers; Harry R Büller; Henri H Versteeg; Max Nieuwdorp; Thijs E van Mens Journal: J Thromb Haemost Date: 2019-12-27 Impact factor: 5.824
Authors: Genevieve L Y Rocheleau; Terry Lee; Yassene Mohammed; David Goodlett; Kevin Burns; Matthew P Cheng; Karen Tran; David Sweet; John Marshall; Arthur S Slutsky; Srinivas Murthy; Joel Singer; David M Patrick; Bin Du; Zhiyong Peng; Todd C Lee; John H Boyd; Keith R Walley; Francois Lamontagne; Robert Fowler; Brent W Winston; Greg Haljan; Donald C Vinh; Alison McGeer; David Maslove; Santiago Perez Patrigeon; Puneet Mann; Kathryn Donohoe; Geraldine Hernandez; James A Russell Journal: Crit Care Med Date: 2022-05-18 Impact factor: 9.296
Authors: Yassene Mohammed; Carolina E Touw; Banne Nemeth; Raymond A van Adrichem; Christoph H Borchers; Frits R Rosendaal; Bart J van Vlijmen; Suzanne C Cannegieter Journal: J Thromb Haemost Date: 2022-01-06 Impact factor: 16.036