Zaixian Zhang1, Yong Hu2, Jia Yang1, Yanhong Xu1, Chengzhong Zhang1, Zhongling Wang1, Xiangyang Shi3, Guixiang Zhang4. 1. Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. 2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. 3. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. xshi@dhu.edu.cn. 4. Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. guixiangzhang@sina.com.
Abstract
PURPOSE: The purpose of this study was to develop folic acid (FA)-modified iron oxide (Fe3O4) nanoparticles (NPs) for targeted magnetic resonance imaging (MRI) of H460 lung carcinoma cells. PROCEDURES: Water-dispersible Fe3O4 NPs synthesized via a mild reduction method were conjugated with FA to generate FA-targeted Fe3O4 NPs. The specificity of FA-targeted Fe3O4 NPs to bind FA receptor was investigated in vitro by cellular uptake and cell MRI and in vivo by MRI of H460 tumors. RESULTS: The formed NPs displayed good biocompatibility and ultrahigh r 2 relaxivity (440.01/mM/s). The targeting effect of the NPs to H460 cells was confirmed by in vitro cellular uptake and cell MRI. H460 tumors showed a significant reduction in T2 signal intensity at 0.85 h, which then recovered and returned to control at 2.35 h. CONCLUSIONS: The results indicate that the prepared FA-targeted Fe3O4 NPs have potential to be used as T2 negative contrast agents in targeted MRI.
PURPOSE: The purpose of this study was to develop folic acid (FA)-modified iron oxide (Fe3O4) nanoparticles (NPs) for targeted magnetic resonance imaging (MRI) of H460 lung carcinoma cells. PROCEDURES: Water-dispersible Fe3O4 NPs synthesized via a mild reduction method were conjugated with FA to generate FA-targeted Fe3O4 NPs. The specificity of FA-targeted Fe3O4 NPs to bind FA receptor was investigated in vitro by cellular uptake and cell MRI and in vivo by MRI of H460 tumors. RESULTS: The formed NPs displayed good biocompatibility and ultrahigh r 2 relaxivity (440.01/mM/s). The targeting effect of the NPs to H460 cells was confirmed by in vitro cellular uptake and cell MRI. H460 tumors showed a significant reduction in T2 signal intensity at 0.85 h, which then recovered and returned to control at 2.35 h. CONCLUSIONS: The results indicate that the prepared FA-targeted Fe3O4 NPs have potential to be used as T2 negative contrast agents in targeted MRI.
Entities:
Keywords:
FA; H460 cells; Iron oxide nanoparticles; MRI
Authors: Mehdi Azizi; Hassan Dianat-Moghadam; Roya Salehi; Masoud Farshbaf; Disha Iyengar; Samaresh Sau; Arun K Iyer; Hadi Valizadeh; Mohammad Mehrmohammadi; Michael R Hamblin Journal: Adv Funct Mater Date: 2020-03-03 Impact factor: 18.808