Virginia Campani1, Marco Biondi2, Laura Mayol3, Francesco Cilurzo4, Silvia Franzé5, Michele Pitaro6, Giuseppe De Rosa7. 1. Department of Pharmacy, Università degli Studi di Napoli Federico II, Via Domenico Montesano 49, 80131, Naples, Italy. virginia.campani@unina.it. 2. Department of Pharmacy, Università degli Studi di Napoli Federico II, Via Domenico Montesano 49, 80131, Naples, Italy. mabiondi@unina.it. 3. Department of Pharmacy, Università degli Studi di Napoli Federico II, Via Domenico Montesano 49, 80131, Naples, Italy. laumayol@unina.it. 4. Department of Pharmaceutical Sciences, Università di Milano, Milan, 20133, Italy. francesco.cilurzo@unimi.it. 5. Department of Pharmaceutical Sciences, Università di Milano, Milan, 20133, Italy. silvia.franze@unimi.it. 6. XenusSrl, Rome, 00173, Italy. michele.pitaro@xenuspharma.it. 7. Department of Pharmacy, Università degli Studi di Napoli Federico II, Via Domenico Montesano 49, 80131, Naples, Italy. gderosa@unina.it.
Abstract
PURPOSE: Vitamin K1 (VK1) is a molecule abundant in some species of leaf vegetables with beneficial effects in humans following administration on the skin. This work investigates the possibility to use formulations based on lipid vesicles, namely liposomes, transfersomes and ethosomes, suitable to be administered on the skin by nebulization and alternative to fat semisolid preparations present on the market. METHODS: Lipid vesicles encapsulating VK1 were prepared and characterized. Ex-vivo experiments on Franz cells were carried out to study the VK1 accumulation/permeation in/through the skin. Vesicles interaction with the skin was investigated by confocal laser scanning microscopy (CLSM) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. RESULTS: All developed carriers were stable following long-term storage and were not altered following nebulization. In ex-vivo experiments, vesicles with the highest deformability index, namely transfersomes and ethosomes, led to an enhanced VK1 accumulation/permeation into/through the skin. Interestingly, the nebulization of the vesicles led to a further increase of VK1 accumulation into the skin. CONCLUSIONS: In conclusion, to achieve a local effect of VK1 on the skin, the topical nebulization of VK1-containing transfersomes could offer a good compromise between a high VK1 penetration into the skin and a limited permeation through it.
PURPOSE:Vitamin K1 (VK1) is a molecule abundant in some species of leaf vegetables with beneficial effects in humans following administration on the skin. This work investigates the possibility to use formulations based on lipid vesicles, namely liposomes, transfersomes and ethosomes, suitable to be administered on the skin by nebulization and alternative to fat semisolid preparations present on the market. METHODS:Lipid vesicles encapsulating VK1 were prepared and characterized. Ex-vivo experiments on Franz cells were carried out to study the VK1 accumulation/permeation in/through the skin. Vesicles interaction with the skin was investigated by confocal laser scanning microscopy (CLSM) and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. RESULTS: All developed carriers were stable following long-term storage and were not altered following nebulization. In ex-vivo experiments, vesicles with the highest deformability index, namely transfersomes and ethosomes, led to an enhanced VK1 accumulation/permeation into/through the skin. Interestingly, the nebulization of the vesicles led to a further increase of VK1 accumulation into the skin. CONCLUSIONS: In conclusion, to achieve a local effect of VK1 on the skin, the topical nebulization of VK1-containing transfersomes could offer a good compromise between a high VK1 penetration into the skin and a limited permeation through it.
Entities:
Keywords:
Franz cells; lipid-vesicles; nebulization; skin; vitamin K1
Authors: Ana Luiza M da Silva; Renata V Contri; Denise S Jornada; Adriana R Pohlmann; Silvia S Guterres Journal: Skin Res Technol Date: 2012-06-07 Impact factor: 2.365
Authors: Virginia Campani; Lorena Scotti; Teresa Silvestri; Marco Biondi; Giuseppe De Rosa Journal: J Mater Sci Mater Med Date: 2020-01-21 Impact factor: 3.896
Authors: Michele Schlich; Umberto M Musazzi; Virginia Campani; Marco Biondi; Silvia Franzé; Francesco Lai; Giuseppe De Rosa; Chiara Sinico; Francesco Cilurzo Journal: Drug Deliv Transl Res Date: 2021-11-09 Impact factor: 5.671