BACKGROUND: The incorporation of substances in nanocarriers can modulate and/or manage their delivery profiles (immediate or sustained) and permeation through skin. Consequently, drug nanencapsulation intended for topical treatment can reduce the systemic absorption of the substance. OBJECTIVE: To obtain and characterize vitamin K1-loaded lipid core nanocapsules as well as to determine whether the nanoencapsulation influences the skin permeation of this vitamin. METHODS: The skin permeation study was performed by means of Franz-type diffusion cells followed by the tape stripping and retention techniques. The vitamin K1-loaded lipid core nanocapsules were obtained by the preformed polymer precipitation method and the particles were characterized. RESULTS: The nanocapsules presented average diameter of 211 ± 2 nm, pH of 5.7 ± 0.3, zeta potential of -14.9 ± 0.6 mV and drug content of 10.2 mg/mL (102.1%). The physical stability of the nanocapsule suspension was verified using multiple light backscattering analysis. The amount of vitamin K1 in the dermis after 8 h of drug permeation was higher when the nanocapsules were applied compared to the control. Moreover, retention in the outermost skin layer and a decrease in the skin permeation to the receptor compartment due to the nanoencapsulation were observed. CONCLUSION: Thus, nanoencapsulation can lead to the selective permeation of vitamin K1 through the skin.
BACKGROUND: The incorporation of substances in nanocarriers can modulate and/or manage their delivery profiles (immediate or sustained) and permeation through skin. Consequently, drug nanencapsulation intended for topical treatment can reduce the systemic absorption of the substance. OBJECTIVE: To obtain and characterize vitamin K1-loaded lipid core nanocapsules as well as to determine whether the nanoencapsulation influences the skin permeation of this vitamin. METHODS: The skin permeation study was performed by means of Franz-type diffusion cells followed by the tape stripping and retention techniques. The vitamin K1-loaded lipid core nanocapsules were obtained by the preformed polymer precipitation method and the particles were characterized. RESULTS: The nanocapsules presented average diameter of 211 ± 2 nm, pH of 5.7 ± 0.3, zeta potential of -14.9 ± 0.6 mV and drug content of 10.2 mg/mL (102.1%). The physical stability of the nanocapsule suspension was verified using multiple light backscattering analysis. The amount of vitamin K1 in the dermis after 8 h of drug permeation was higher when the nanocapsules were applied compared to the control. Moreover, retention in the outermost skin layer and a decrease in the skin permeation to the receptor compartment due to the nanoencapsulation were observed. CONCLUSION: Thus, nanoencapsulation can lead to the selective permeation of vitamin K1 through the skin.
Authors: Virginia Campani; Marco Biondi; Laura Mayol; Francesco Cilurzo; Silvia Franzé; Michele Pitaro; Giuseppe De Rosa Journal: Pharm Res Date: 2015-11-30 Impact factor: 4.200
Authors: Pierre P D Kondiah; Thankhoe A Rants'o; Sipho Mdanda; Lauwrence M Mohlomi; Yahya E Choonara Journal: Polymers (Basel) Date: 2022-06-28 Impact factor: 4.967
Authors: João Guilherme B De Marchi; Denise S Jornada; Fernanda K Silva; Ana L Freitas; Alexandre M Fuentefria; Adriana R Pohlmann; Silvia S Guterres Journal: Int J Nanomedicine Date: 2017-10-25
Authors: Virginia Campani; Dario Marchese; Maria Teresa Pitaro; Michele Pitaro; Paolo Grieco; Giuseppe De Rosa Journal: Int J Nanomedicine Date: 2014-04-10