Stefanie Schienle1, Ali Al-Ahmad1, Ralf Joachim Kohal2, Falk Bernsmann3, Erik Adolfsson4, Laura Montanaro5, Paola Palmero5, Tobias Fürderer6, Jérôme Chevalier7, Elmar Hellwig1, Lamprini Karygianni8. 1. Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert Ludwigs University, Hugstetter Strasse 55, 79106, Freiburg, Germany. 2. Division of Prosthodontics, Department of Dental Medicine, Oral and Maxillofacial Surgery, Medical Center, University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany. 3. NTTF Coatings GmbH, Maarweg 32, 53619, Rheinbreitbach, Germany. 4. Swerea IVF AB, Argongatan 30, 43153, Mölndal, Sweden. 5. Politecnico di Torino, Department of Applied Science and Technology, INSTM RU Politecnico di Torino, LINCE Lab, Corso Duca degli Abruzzi, Turin, Italy. 6. DOCERAM Medical Ceramics GmbH, Hesslingsweg 65-67, 44309, Dortmund, Germany. 7. Université de Lyon, INSA-Lyon MATEIS UMR CNRS 5510, Bât. Blaise Pascal 7 Av. Jean Capelle, 69621, Villeurbanne, France. 8. Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Albert Ludwigs University, Hugstetter Strasse 55, 79106, Freiburg, Germany. lamprini.karygianni@uniklinik-freiburg.de.
Abstract
OBJECTIVES: Biomaterial surfaces are at high risk for initial microbial colonization, persistence, and concomitant infection. The rationale of this study was to assess the initial adhesion on novel implant surfaces of Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans upon incubation. MATERIALS AND METHODS: The tested samples were 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) samples with nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) coating (A) and 3Y-TZP samples coated with ceria-stabilized zirconia-based (Ce-TZP) composite and a-C:H:N (B). Uncoated 3Y-TZP samples (C) and bovine enamel slabs (BES) served as controls. Once the surface was characterized, the adherent microorganisms were quantified by estimating the colony-forming units (CFUs). Microbial vitality was assessed by live/dead staining, and microbial-biomaterial surface topography was visualized by scanning electron microscopy (SEM). RESULTS: Overall, A and B presented the lowest CFU values for all microorganisms, while C sheltered significantly less E. faecalis, P. aeruginosa, and C. albicans than BES. Compared to the controls, B demonstrated the lowest vitality values for E. coli (54.12 %) and C. albicans (67.99 %). Interestingly, A (29.24 %) exhibited higher eradication rates for S. aureus than B (13.95 %). CONCLUSIONS: Within the limitations of this study, a-C:H:N-coated 3Y-TZP surfaces tended to harbor less initially adherent microorganisms and selectively interfered with their vitality. CLINICAL RELEVANCE: This could enable further investigation of the new multi-functional zirconia surfaces to confirm their favorable antimicrobial properties in vivo.
OBJECTIVES: Biomaterial surfaces are at high risk for initial microbial colonization, persistence, and concomitant infection. The rationale of this study was to assess the initial adhesion on novel implant surfaces of Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans upon incubation. MATERIALS AND METHODS: The tested samples were 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) samples with nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) coating (A) and 3Y-TZP samples coated with ceria-stabilized zirconia-based (Ce-TZP) composite and a-C:H:N (B). Uncoated 3Y-TZP samples (C) and bovine enamel slabs (BES) served as controls. Once the surface was characterized, the adherent microorganisms were quantified by estimating the colony-forming units (CFUs). Microbial vitality was assessed by live/dead staining, and microbial-biomaterial surface topography was visualized by scanning electron microscopy (SEM). RESULTS: Overall, A and B presented the lowest CFU values for all microorganisms, while C sheltered significantly less E. faecalis, P. aeruginosa, and C. albicans than BES. Compared to the controls, B demonstrated the lowest vitality values for E. coli (54.12 %) and C. albicans (67.99 %). Interestingly, A (29.24 %) exhibited higher eradication rates for S. aureus than B (13.95 %). CONCLUSIONS: Within the limitations of this study, a-C:H:N-coated 3Y-TZP surfaces tended to harbor less initially adherent microorganisms and selectively interfered with their vitality. CLINICAL RELEVANCE: This could enable further investigation of the new multi-functional zirconia surfaces to confirm their favorable antimicrobial properties in vivo.
Authors: Fernanda H Schünemann; María E Galárraga-Vinueza; Ricardo Magini; Márcio Fredel; Filipe Silva; Júlio C M Souza; Yu Zhang; Bruno Henriques Journal: Mater Sci Eng C Mater Biol Appl Date: 2019-01-16 Impact factor: 7.328