Jae-Sung Kwon1, Yong Hee Kim2, Eun Ha Choi2, Chong-Kwan Kim3, Kyoung-Nam Kim1, Kwang-Mahn Kim4. 1. BK21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea. 2. Plasma Bioscience Research Center, Kwangwoon University, Seoul, Republic of Korea. 3. Department of Periodontology, Yonsei University College of Dentistry, Seoul, Republic of Korea. 4. BK21 PLUS Project, Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea. kmkim@yuhs.ac.
Abstract
OBJECTIVES: The aim of this in vitro study was to investigate the effects of a non-thermal atmospheric pressure plasma jet (NTAPPJ) on the cellular activity of human gingival fibroblasts (HGF) for possible non-surgical application of it during gingival wound healing. MATERIALS AND METHODS: HGF cells were exposed with NTAPPJ for 1, 2, and 4 min and were investigated for cellular attachment, cell viability, morphology of attached cells, proliferation rate, and messenger ribonucleic acid (mRNA) expression of various growth factors. Also, scavengers for chemicals produced by NTAPPJ were used to identify the chemical species responsible for the effects. RESULTS: There was no significant change in the number of HGF cells attached or their proliferation following NTAPPJ exposure. Also, high cell viability resulted from exposure of all of HGF cells to NTAPPJ for 1, 2, and 4 min. However, cells were more stretched while the mRNA expressions of transforming growth factor and vascular endothelial growth factor were significantly increased following NTAPPJ exposure. Additionally, the scavenger test showed that nitric oxide is likely to be the chemical responsible for an increase of cellular activity. CONCLUSION: The results demonstrated that the NTAPPJ increased mRNA expressions of growth factors in human gingival fibroblasts. CLINICAL RELEVANCE: Application of NTAPPJ would be useful in gingival wound healing in clinics though additional studies confirming the effects would be needed.
OBJECTIVES: The aim of this in vitro study was to investigate the effects of a non-thermal atmospheric pressure plasma jet (NTAPPJ) on the cellular activity of human gingival fibroblasts (HGF) for possible non-surgical application of it during gingival wound healing. MATERIALS AND METHODS:HGF cells were exposed with NTAPPJ for 1, 2, and 4 min and were investigated for cellular attachment, cell viability, morphology of attached cells, proliferation rate, and messenger ribonucleic acid (mRNA) expression of various growth factors. Also, scavengers for chemicals produced by NTAPPJ were used to identify the chemical species responsible for the effects. RESULTS: There was no significant change in the number of HGF cells attached or their proliferation following NTAPPJ exposure. Also, high cell viability resulted from exposure of all of HGF cells to NTAPPJ for 1, 2, and 4 min. However, cells were more stretched while the mRNA expressions of transforming growth factor and vascular endothelial growth factor were significantly increased following NTAPPJ exposure. Additionally, the scavenger test showed that nitric oxide is likely to be the chemical responsible for an increase of cellular activity. CONCLUSION: The results demonstrated that the NTAPPJ increased mRNA expressions of growth factors in human gingival fibroblasts. CLINICAL RELEVANCE: Application of NTAPPJ would be useful in gingival wound healing in clinics though additional studies confirming the effects would be needed.
Authors: Na Yu; Daniel A W Oortgiesen; Antonius L J J Bronckers; Fang Yang; X Frank Walboomers; John A Jansen Journal: J Clin Periodontol Date: 2013-05-15 Impact factor: 8.728
Authors: K Tomikawa; T Yamamoto; N Shiomi; M Shimoe; S Hongo; K Yamashiro; T Yamaguchi; H Maeda; S Takashiba Journal: J Dent Res Date: 2012-06-14 Impact factor: 6.116
Authors: Anna T Grazul-Bilska; Mary Lynn Johnson; Jerzy J Bilski; Dale A Redmer; Lawrence P Reynolds; Ahmed Abdullah; Kay M Abdullah Journal: Drugs Today (Barc) Date: 2003-10 Impact factor: 2.245
Authors: Benedikt Eggers; Matthias Bernhard Stope; Jana Marciniak; Werner Götz; Alexander Mustea; James Deschner; Marjan Nokhbehsaim; Franz-Josef Kramer Journal: Biomedicines Date: 2022-04-13