B A Pedersen1, P G Yazdi2, J F Taylor3, O S Khattab4, Y-H Chen3, Y Chen3, P H Wang5. 1. UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA. 2. UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Systomic Health LLC, Los Angeles, CA 90039, USA. 3. UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA. 4. UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA. 5. UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA; Sue and Bill Gross Stem Cell Research Center, University of California at Irvine, Irvine, CA 92697, USA; Department of Medicine, University of California at Irvine, Irvine, CA 92697, USA; Department of Biological Chemistry, University of California at Irvine, Irvine, CA 92697, USA; Department of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA. Electronic address: phwang@uci.edu.
Abstract
BACKGROUND AND AIM: Mitochondrial dysfunction contributes to the pathophysiology of diabetic cardiomyopathy. The aim of this study was to investigate the acute changes in the mitochondrial proteome in response to insulin stimulation. METHODS AND RESULTS: Cardiac mitochondria from C57BL/6 mice after insulin stimulation were analyzed using two-dimensional fluorescence difference gel electrophoresis. MALDI-TOF MS/MS was utilized to identify differences. Two enzymes involved in metabolism and four structural proteins were identified. Succinyl-CoA ligase [ADP forming] subunit beta was identified as one of the differentially regulated proteins. Upon insulin stimulation, a relatively more acidic isoform of this protein was increased by 53% and its functional activity was decreased by ∼32%. CONCLUSIONS: This proteomic remodeling in response to insulin stimulation may play an important role in the normal and diabetic heart.
BACKGROUND AND AIM: Mitochondrial dysfunction contributes to the pathophysiology of diabetic cardiomyopathy. The aim of this study was to investigate the acute changes in the mitochondrial proteome in response to insulin stimulation. METHODS AND RESULTS: Cardiac mitochondria from C57BL/6 mice after insulin stimulation were analyzed using two-dimensional fluorescence difference gel electrophoresis. MALDI-TOF MS/MS was utilized to identify differences. Two enzymes involved in metabolism and four structural proteins were identified. Succinyl-CoA ligase [ADP forming] subunit beta was identified as one of the differentially regulated proteins. Upon insulin stimulation, a relatively more acidic isoform of this protein was increased by 53% and its functional activity was decreased by ∼32%. CONCLUSIONS: This proteomic remodeling in response to insulin stimulation may play an important role in the normal and diabetic heart.
Authors: R Prabhakar; G P Boivin; I L Grupp; B Hoit; G Arteaga; R J Solaro; D F Wieczorek Journal: J Mol Cell Cardiol Date: 2001-10 Impact factor: 5.000
Authors: Vamsi K Mootha; Jakob Bunkenborg; Jesper V Olsen; Majbrit Hjerrild; Jacek R Wisniewski; Erich Stahl; Marjan S Bolouri; Heta N Ray; Smita Sihag; Michael Kamal; Nick Patterson; Eric S Lander; Matthias Mann Journal: Cell Date: 2003-11-26 Impact factor: 41.582
Authors: J F Glatz; E van Breda; H A Keizer; Y F de Jong; J R Lakey; R V Rajotte; A Thompson; G J van der Vusse; G D Lopaschuk Journal: Biochem Biophys Res Commun Date: 1994-03-15 Impact factor: 3.575