H Tolba1, H Moghrani2, A Benelmouffok3, D Kellou3, R Maachi2. 1. Laboratory of reaction engineering, department of process engineering, faculty of mechanical engineering and process engineering, USTHB, PO Box 32 El Alia, Bab Ezzouar, 16111 Algiers, Algeria. Electronic address: htolba@usthb.dz. 2. Laboratory of reaction engineering, department of process engineering, faculty of mechanical engineering and process engineering, USTHB, PO Box 32 El Alia, Bab Ezzouar, 16111 Algiers, Algeria. 3. Laboratory of Mycology, Pasteur Institute, Algiers, Algeria.
Abstract
OBJECTIVE: Essential oil of Eucalyptus citriodora is a natural product which has been attributed for various medicinal uses. In the present investigation, E. citriodora essential oil was used to evaluate its antifungal effect against medically important dermatophytes. MATERIAL AND METHODS: Essential oil from the Algerian E. citriodora leaves was analyzed by GC and GC/MS. The antifungal effect of E. citriodora essential oil was evaluated against four dermatophytes: Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum using disc diffusion method, disc volatilization method, and agar dilution method. RESULTS: The chemical composition of the oil revealed the presence of 22 compounds accounting for 95.27% of the oil. The dominant compounds were citronellal (69.77%), citronellol (10.63%) and isopulegol (4.66%). The disc diffusion method, MIC and MFC determination, indicated that E. citriodora essential oil had a higher antifungal potential against the tested strains with inhibition zone diameter which varied from (12 to 90mm) and MIC and MFC values ranged from (0.6 to 5μL/mL and 1.25 to 5μL/mL) respectively. The M. gypseum was the most resistant to the oil. CONCLUSION: The results of the present study indicated that E. citriodora essential oil may be used as a new antifungal agent recommended by the pharmaceutical industries.
OBJECTIVE:Essential oil of Eucalyptus citriodora is a natural product which has been attributed for various medicinal uses. In the present investigation, E. citriodora essential oil was used to evaluate its antifungal effect against medically important dermatophytes. MATERIAL AND METHODS:Essential oil from the Algerian E. citriodora leaves was analyzed by GC and GC/MS. The antifungal effect of E. citriodora essential oil was evaluated against four dermatophytes: Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum using disc diffusion method, disc volatilization method, and agar dilution method. RESULTS: The chemical composition of the oil revealed the presence of 22 compounds accounting for 95.27% of the oil. The dominant compounds were citronellal (69.77%), citronellol (10.63%) and isopulegol (4.66%). The disc diffusion method, MIC and MFC determination, indicated that E. citriodora essential oil had a higher antifungal potential against the tested strains with inhibition zone diameter which varied from (12 to 90mm) and MIC and MFC values ranged from (0.6 to 5μL/mL and 1.25 to 5μL/mL) respectively. The M. gypseum was the most resistant to the oil. CONCLUSION: The results of the present study indicated that E. citriodora essential oil may be used as a new antifungal agent recommended by the pharmaceutical industries.
Authors: Cornelia Vasile; Morten Sivertsvik; Amalia Carmen Miteluţ; Mihai Adrian Brebu; Elena Stoleru; Jan Thomas Rosnes; Elisabeta Elena Tănase; Waqas Khan; Daniela Pamfil; Călina Petruţa Cornea; Anamaria Irimia; Mona Elena Popa Journal: Materials (Basel) Date: 2017-01-07 Impact factor: 3.623
Authors: Luciani Gaspar De Toledo; Matheus Aparecido Dos Santos Ramos; Larissa Spósito; Elza Maria Castilho; Fernando Rogério Pavan; Érica De Oliveira Lopes; Guilherme Julião Zocolo; Francisca Aliny Nunes Silva; Tigressa Helena Soares; André Gonzaga Dos Santos; Taís Maria Bauab; Margarete Teresa Gottardo De Almeida Journal: Int J Mol Sci Date: 2016-08-09 Impact factor: 5.923