Literature DB >> 26584920

Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM).

Ryohei Narumi1,2, Takeshi Tomonaga3.   

Abstract

Mass spectrometry-based phosphoproteomics is an indispensible technique used in the discovery and quantification of phosphorylation events on proteins in biological samples. The application of this technique to tissue samples is especially useful for the discovery of biomarkers as well as biological studies. We herein describe the application of a large-scale phosphoproteome analysis and SRM/MRM-based quantitation to develop a strategy for the systematic discovery and validation of biomarkers using tissue samples.

Keywords:  IMAC; MRM; Phosphoproteome; SRM; iTRAQ

Mesh：

Substances：

Year:  2016        PMID： 26584920     DOI： 10.1007/978-1-4939-3049-4_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins.

Authors:  Ryohei Narumi; Yoshihiro Shimizu; Maki Ukai-Tadenuma; Koji L Ode; Genki N Kanda; Yuta Shinohara; Aya Sato; Katsuhiko Matsumoto; Hiroki R Ueda
Journal:  Proc Natl Acad Sci U S A       Date:  2016-05-31       Impact factor: 11.205

2.  Increased serum protein levels by Yuanshi Shengmai Chenggu Tablet in treatment of avascular osteonecrosis of the femoral head.

Authors:  Jianchun Zeng; Peng Deng; Jie Li; Wenjun Feng; Jinlun Chen; Yirong Zeng
Journal:  Mol Med Rep       Date:  2017-11-20       Impact factor: 2.952

Review 3.  The Human Skeletal Muscle Proteome Project: a reappraisal of the current literature.

Authors:  Marta Gonzalez-Freire; Richard D Semba; Ceereena Ubaida-Mohien; Elisa Fabbri; Paul Scalzo; Kurt Højlund; Craig Dufresne; Alexey Lyashkov; Luigi Ferrucci
Journal:  J Cachexia Sarcopenia Muscle       Date:  2016-08-03       Impact factor: 12.910

Review 4.  Proteomics progresses in microbial physiology and clinical antimicrobial therapy.

Authors:  B Chen; D Zhang; X Wang; W Ma; S Deng; P Zhang; H Zhu; N Xu; S Liang
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-11-04       Impact factor: 3.267

5.  Screening of Serum Protein Markers for Avascular Osteonecrosis of Femoral Head Differentially Expressed after Treatment with Yuanshi Shengmai Chenggu Tablets.

Authors:  Peng Deng; Jianchun Zeng; Jie Li; Wenjun Feng; Jinlun Chen; Yirong Zeng
Journal:  Biomed Res Int       Date:  2018-03-20       Impact factor: 3.411
  5 in total

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